PCMT1 Antikörper (Middle Region)

Produktnummer ABIN2773925

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PCMT1 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Kaninchen, Ziege, Pferd und Zebrafisch (Danio rerio).

Kurzübersicht für PCMT1 Antikörper (Middle Region) (ABIN2773925)

Target: PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Kaninchen, Ziege, Pferd, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCMT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PCMT1 Antikörper

Bindungsspezifität: Middle Region

Sequenz: APYDAIHVGA AAPVVPQALI DQLKPGGRLI LPVGPAGGNQ MLEQYDKLQD

Homologie: Cow: 100%, Dog: 100%, Goat: 79%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against PCMT1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PCMT1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 227 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu PCMT1

Target: PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))

Andere Bezeichnung: PCMT1

Hintergrund: Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: PIMT
Protein Interaction Partner: RPIA, HUWE1, AIMP2, TP53, AURKA, UBC, SUMO1, NEDD8, SH3RF2, LMNA, VCAM1, ITGA4, FN1, CFTR, VKORC1, TP53BP1, SDHAF2, NANS, UBE2C, SEC24A, PPIH, ZRANB2, UCHL3, TPM4, SVIL, RRM2, RAD23A, PSMB7, PFDN2, NOTCH2, GDI1, ATP6V1B2, APOE, CUL1, CUL2, CUL3, CDK2, CAN
Protein Size: 227

Molekulargewicht: 25 kDa

Gen-ID: 5110

NCBI Accession: NM_005389, NP_005380

UniProt: P22061

Pathways: Ribonucleoprotein Complex Subunit Organization