Topoisomerase I Antikörper

Produktnummer ABIN2763824

Der Kaninchen Polyklonal Anti-Topoisomerase I-Antikörper wurde für IHC (p) validiert. Er ist geeignet, Topoisomerase I in Proben von Human zu detektieren.

Kurzübersicht für Topoisomerase I Antikörper (ABIN2763824)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Topoisomerase I Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Topoisomerase I Antikörper

Kreuzreaktivität: Human

Produktmerkmale: DNA Topoisomerase 1 releases the supercoiling and torsional tension of DNA introduced during DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. It introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Aufreinigung: Antigen Immunoaffinity Purification

Immunogen: Synthetic peptide made to human DNA Topoisomerase 1

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Antibody can be used for immunohistochemistry at 5-10 μg/mL on paraffin-embedded tissues. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: DNA Topoisomerase 1

UniProt: P11387

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance