TPBG Antikörper

Produktnummer ABIN2763610

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch TPBG in WB, ELISA und IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für TPBG Antikörper (ABIN2763610)

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TPBG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-TPBG Antikörper

Kreuzreaktivität: Human

Produktmerkmale: Trophoblast glycoprotein, TPBG, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, 5T4 oncofetal trophoblast glycoprotein, M6P1,5T4 is a N-glycosylated transmembrane 72 kDa (420 AA) glycoprotein containing seven leucine-rich repeat regions. 5T4 is often referred to as an oncofetal antigen due to its expression in fetal trophoblast where it was first discovered or trophoblast glycoprotein (TPBG). 5T4 oncofetal antigen is defined by a monoclonal antibody originally raised against human placental trophoblast and which recognizes a 72 kD glycoprotein expressed in a variety of different carcinomas but detected only at very low levels in normal epithelia. Patterns of expression of 5T4 oncofetal antigen in colorectal carcinomas indicate a significant association with tumor metastasis. By IHC, 71 % of the carcinomas demonstrate positive 5T4 immunoreactivity in adenocarcinoma cells and/or associated stromal tissue.5T4 is found in tumors including the colorectal, ovarian, and gastric. Its expression is used as a prognostic aid in these cases. 5T4 is widespread in malignant tumors throughout the various stages of their development. In assessing the 5T4 phenotype it has been determined that there is a significant correlation between 5T4 expression and advanced stage of disease (FIGO stages III and IV) (P < 0.001) and with poorly differentiated tumors (P = 0.036) compared to well or moderately differentiated tumors. Patients with tumors expressing 5T4 were are likely to respond well to adjuvant therapy and have a significantly worse outlook in terms of survival and disease-free interval.

Aufreinigung: Antigen Immunoaffinity Purification

Immunogen: Synthetic peptide derived from human 5T4 oncofetal antigen protein

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: This antibody can be used for immunohistochemistry on formalin/paraffin sections (5-10 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu TPBG

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Andere Bezeichnung: 5T4 Oncofetal Trophoblast glycoprotein

UniProt: Q13641