Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD) Antikörper

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Antigen
Reaktivität
Arabidopsis thaliana, Chlamydomonas reinhardtii (C. reinhardtii), Reis (Oryza sativa), Maize/Corn (Zea mays)
Wirt
Kaninchen
Klonalität
Polyklonal
Applikation
Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece
Kreuzreaktivität (Details) No cross-reactivity with: cyanobacteria
Produktmerkmale Expected / apparent Molecular Weight of the Antigene: 25 / 22 kDa
Reinigung serum
Hintergrund AGI Code: At4g25100
Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
Molekulargewicht expected: 25 kDa, apparent: 22 kDa
UniProt A8IGH1
Applikationshinweise Recommended Dilution: 1 : 1500 to 1 : 5000 with alkaline phosphatase (WB).
Kommentare

The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 µM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For reconstitution add 200 µL of sterile water.
Handhabung Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Lagerung -20 °C
Bilder des Herstellers
 image for anti-Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD) antibody (ABIN2749232) anti-Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD) antibody
Allgemeine Veröffentlichungen Dang, Plet, Tolleter, Jokel, Cuiné, Carrier, Auroy, Richaud, Johnson, Alric, Allahverdiyeva, Peltier: "Combined increases in mitochondrial cooperation and oxygen photoreduction compensate for deficiency in cyclic electron flow in Chlamydomonas reinhardtii." in: The Plant cell, Vol. 26, Issue 7, pp. 3036-50, 2014 (PubMed).

Produkt verwendet in: Page, Allen, Kropat, Urzica, Karpowicz, Hsieh, Loo, Merchant: "Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii." in: The Plant cell, Vol. 24, Issue 6, pp. 2649-65, 2012 (PubMed).

Abdel-Ghany, Müller-Moulé, Niyogi, Pilon, Shikanai: "Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts." in: The Plant cell, Vol. 17, Issue 4, pp. 1233-51, 2005 (PubMed).

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