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FeSOD Antikörper

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch FeSOD in WB. Er zeigt eine Reaktivität gegenüber Arabidopsis thaliana, Chlamydomonas reinhardtii, Oryza sativa und Mais (Zea mays) und wurde in 2+ Publikationen erwähnt.
Produktnummer ABIN2749232

Kurzübersicht für FeSOD Antikörper (ABIN2749232)

Target

FeSOD (Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD))

Reaktivität

Arabidopsis thaliana, Chlamydomonas reinhardtii, Oryza sativa, Mais (Zea mays)

Wirt

Kaninchen

Klonalität

Polyklonal

Applikation

Western Blotting (WB)
  • Kreuzreaktivität (Details)

    No cross-reactivity with: cyanobacteria

    Produktmerkmale

    Expected / apparent Molecular Weight of the Antigene: 25 / 22 kDa

    Aufreinigung

    serum

    Immunogen

    overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece
  • Applikationshinweise

    Recommended Dilution: 1 : 1500 to 1 : 5000 with alkaline phosphatase (WB).

    Kommentare

    The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 µM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 200 µL of sterile water.

    Handhabung

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Lagerung

    -20 °C
  • Page, Allen, Kropat, Urzica, Karpowicz, Hsieh, Loo, Merchant: "Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii." in: The Plant cell, Vol. 24, Issue 6, pp. 2649-65, (2012) (PubMed).

    Abdel-Ghany, Müller-Moulé, Niyogi, Pilon, Shikanai: "Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts." in: The Plant cell, Vol. 17, Issue 4, pp. 1233-51, (2005) (PubMed).

  • Target

    FeSOD (Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD))

    Hintergrund

    AGI Code: At4g25100
    Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

    Molekulargewicht

    expected: 25 kDa, apparent: 22 kDa

    UniProt

    A8IGH1
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