MCAM Antikörper (PE)

Produktnummer ABIN2749196

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MCAM in FACS. Er zeigt eine Reaktivität gegenüber Human, Maus, Kaninchen und Hund und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für MCAM Antikörper (PE) (ABIN2749196)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reaktivität: Human, Maus, Kaninchen, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCAM Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: P1H12

Produktdetails anti-MCAM Antikörper (PE)

Verwendungszweck: Anti-Hu CD146 PE

Spezifität: The mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.

Keine Kreuzreaktivität: Ratte

Kreuzreaktivität (Details): Human, Mouse, Canine (Dog), Rabbit

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: cultured human umbilical cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-MCAM Antikörper (PE) (ABIN2749196)

Kamstock, Guth, Elmslie, Kurzman, Liggitt, Coro, Fairman, Dow: "Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma." in: Cancer gene therapy, Vol. 13, Issue 3, pp. 306-17, (2006) (PubMed).

Solovey, Gui, Chang, Enenstein, Browne, Hebbel: "Identification and functional assessment of endothelial P1H12." in: The Journal of laboratory and clinical medicine, Vol. 138, Issue 5, pp. 322-31, (2001) (PubMed).

Solovey, Lin, Browne, Choong, Wayner, Hebbel: "Circulating activated endothelial cells in sickle cell anemia." in: The New England journal of medicine, Vol. 337, Issue 22, pp. 1584-90, (1997) (PubMed).

Details zu MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Andere Bezeichnung: CD146

Hintergrund: Melanoma cell adhesion molecule,CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50 % of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.,MelCAM, MCAM, MUC18

Gen-ID: 4162

UniProt: P43121