MCAM Antikörper

Produktnummer ABIN2749195

Dieser Anti-MCAM Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von MCAM in FACS, WB, IP, IHC (p), ICC und IHC (fro). Geeignet für Human, Maus, Kaninchen und Hund. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für MCAM Antikörper (ABIN2749195)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reaktivität: Human, Maus, Kaninchen, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCAM Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: P1H12

Produktdetails anti-MCAM Antikörper

Verwendungszweck: Anti-Hu CD146 Purified

Spezifität: The mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.

Keine Kreuzreaktivität: Ratte

Kreuzreaktivität (Details): Human, Mouse, Canine (Dog), Rabbit

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: cultured human umbilical cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting: Nonreducing conditions.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-MCAM Antikörper (ABIN2749195)

Kamstock, Guth, Elmslie, Kurzman, Liggitt, Coro, Fairman, Dow: "Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma." in: Cancer gene therapy, Vol. 13, Issue 3, pp. 306-17, (2006) (PubMed).

Solovey, Gui, Chang, Enenstein, Browne, Hebbel: "Identification and functional assessment of endothelial P1H12." in: The Journal of laboratory and clinical medicine, Vol. 138, Issue 5, pp. 322-31, (2001) (PubMed).

Solovey, Lin, Browne, Choong, Wayner, Hebbel: "Circulating activated endothelial cells in sickle cell anemia." in: The New England journal of medicine, Vol. 337, Issue 22, pp. 1584-90, (1997) (PubMed).

Details zu MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Andere Bezeichnung: CD146

Hintergrund: Melanoma cell adhesion molecule,CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50 % of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.,MelCAM, MCAM, MUC18

Gen-ID: 4162

UniProt: P43121