Fc gamma RII (CD32) Antikörper (PE)

Produktnummer ABIN2749126

Dieses Anti--Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von in FACS. Geeignet für Human. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für Fc gamma RII (CD32) Antikörper (PE) (ABIN2749126)

Target: Fc gamma RII (CD32)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: PE

Applikation: Flow Cytometry (FACS)

Klon: 3D3

Produktdetails

Verwendungszweck: Anti-Hu CD32 PE

Spezifität: The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: purified glycosylated recombinant human FcgammaRIIa2

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen

Os, Bürgler, Ribes, Funderud, Wang, Thompson, Tjønnfjord, Bogen, Munthe: "Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells." in: Cell reports, Vol. 4, Issue 3, pp. 566-77, (2013) (PubMed).

Dai, Jayapal, Tay, Reghunathan, Lin, Too, Lim, Chan, Kemeny, Floto, Smith, Melendez, MacAry: "Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa." in: Blood, Vol. 114, Issue 2, pp. 318-27, (2009) (PubMed).

Dutertre, Bonnin-Gélizé, Pulford, Bourel, Fridman, Teillaud: "A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function." in: Journal of leukocyte biology, Vol. 84, Issue 6, pp. 1511-20, (2008) (PubMed).

Boruchov, Heller, Veri, Bonvini, Ravetch, Young: "Activating and inhibitory IgG Fc receptors on human DCs mediate opposing functions." in: The Journal of clinical investigation, Vol. 115, Issue 10, pp. 2914-23, (2005) (PubMed).

Vely, Gruel, Moncuit, Cochet, Rouard, Dare, Galon, Sautes, Fridman, Teillaud: "A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays." in: Hybridoma, Vol. 16, Issue 6, pp. 519-28, (1998) (PubMed).

Antigendetails

Target: Fc gamma RII (CD32)

Andere Bezeichnung: CD32

Hintergrund: Fc fragment of IgG receptor IIa,CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).,FCG2, FCGR2A, IGFR2

Gen-ID: 2212

UniProt: P12318