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Cytokeratin 19 Antikörper (FITC)

Name: Cytokeratin 19 Antikörper (FITC)
Brand: antibodies-online
SKU: ABIN2749080
Price: 434.15 EUR
Availability: InStock

KRT19 Reaktivität: Human FACS Wirt: Maus Monoclonal A53-B-A2 FITC

Western Blotting analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. (Cytokeratin 19 Antikörper (FITC))

Separation of MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) FITC antibody (concentration in sample 3 μg/mL, red-filled) from MCF-7 cells stained using mouse IgG2b isotype control (MPC-11) FITC antibody (concentration in sample 3 μg/mL, same as anti-Cytokeratin 19 FITC antibody concentration, black-dashed) in flow cytometry analysis (surface staining). (Cytokeratin 19 Antikörper (FITC))

FACS

Separation of MCF-7 human breast adenocarcinoma cell line (red) from human peripheral whole blood (blue) in flow cytometry analysis (intracellular staining) stained using anti-human cytokeratin 19 (A53-B/A2) FITC antibody (concentration in sample 9 μg/mL). (Cytokeratin 19 Antikörper (FITC))

FACS

3 Abbildungen

(2 Referenzen)

Produktnummer ABIN2749080

Datenblatt (PDF)

Target Alle Cytokeratin 19 (KRT19) Antikörper anzeigen

Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
Reaktivität
259

90

72

8

7

7

5

5

4

2

2

2

2

2

2

1

1

1

1

1
Human
Wirt
137

131

3

2

1
Maus
Passende Sekundärantikörper
Klonalität
142

132
Monoklonal
Konjugat
122

33

18

13

7

6

6

5

5

5

5

5

4

4

4

4

4

4

3

3

3

3

3

2

2

1

1
Dieser Cytokeratin 19 Antikörper ist konjugiert mit FITC
Applikation
188

94

84

82

67

45

40

39

33

29

23

22

7

6

6

5

2

1

1

1

1

1

1
Flow Cytometry (FACS)

Spezifität

The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.

Kreuzreaktivität (Details)

Human

Aufreinigung

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen

MCF-7 human breast adenocarcinoma cell line

Klon

A53-B-A2

Isotyp

IgG2a

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Applikationshinweise

Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Kommentare

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Kasper, Moll, Stosiek, Karsten: "Patterns of cytokeratin and vimentin expression in the human eye." in: Histochemistry, Vol. 89, Issue 4, pp. 369-77, (1988) (PubMed).

Karsten, Papsdorf, Roloff, Stolley, Abel, Walther, Weiss: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, (1985) (PubMed).
Target

Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Andere Bezeichnung

Cytokeratin 19 (KRT19 Produkte)

Synonyme

CK19 antikoerper, K19 antikoerper, K1CS antikoerper, AI663979 antikoerper, EndoC antikoerper, Krt-1.19 antikoerper, Krt1-19 antikoerper, Ka19 antikoerper, k19 antikoerper, ck19 antikoerper, k1cs antikoerper, krt9 antikoerper, krt15 antikoerper, MGC76282 antikoerper, GK-19 antikoerper, MGC83069 antikoerper, KRT19 antikoerper, keratin 19 antikoerper, keratin 19 L homeolog antikoerper, keratin, type I cytoskeletal 19 antikoerper, KRT19 antikoerper, Krt19 antikoerper, krt19 antikoerper, krt19.L antikoerper, LOC100344434 antikoerper, LOC101117946 antikoerper

Hintergrund

Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS

Gen-ID

3880

UniProt

P08727