CD4 Antikörper (APC)

Produktnummer ABIN2749063

Produktdetails anti-CD4 Antikörper (APC)

Target: CD4

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Spezifität: The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).

Kreuzreaktivität (Details): Rat

Aufreinigung: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: MLR generated rat Th cells

Klon: OX-35

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-2 μg/mL.

Kommentare:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD4 Antikörper (APC) (ABIN2749063)

Viel, Lemarié, Benkirane, Paradis, Schiffrin: "Immune regulation and vascular inflammation in genetic hypertension." in: American journal of physiology. Heart and circulatory physiology, Vol. 298, Issue 3, pp. H938-44, (2010) (PubMed).

Monzon-Casanova, Steiniger, Schweigle, Clemen, Zdzieblo, Starick, Müller, Wang, Rhost, Cardell, Pyz, Herrmann: "CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation." in: PLoS ONE, Vol. 5, Issue 9, (2010) (PubMed).

Baba, Iwasaki, Maruoka, Suzuki, Tomaru, Ikeda, Yoshiki, Kasahara, Ishizu: "Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D- and granzyme/perforin-dependent mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 5, pp. 2999-3006, (2008) (PubMed).

Ramiro-Puig, Pérez-Cano, Ramos-Romero, Pérez-Berezo, Castellote, Permanyer, Franch, Izquierdo-Pulido, Castell: "Intestinal immune system of young rats influenced by cocoa-enriched diet." in: The Journal of nutritional biochemistry, Vol. 19, Issue 8, pp. 555-65, (2008) (PubMed).

Baba, Ishizu, Iwasaki, Suzuki, Tomaru, Ikeda, Yoshiki, Kasahara: "CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype." in: Blood, Vol. 107, Issue 5, pp. 2004-12, (2006) (PubMed).

Gelderman, Hultqvist, Holmberg, Olofsson, Holmdahl: "T cell surface redox levels determine T cell reactivity and arthritis susceptibility." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 34, pp. 12831-6, (2006) (PubMed).

Yan, Devos, Yu, Xia, Rutgeerts, Goebels, Segers, Lin, Vandeputte, Waer: "Pathogenesis of autoimmunity after xenogeneic thymus transplantation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5936-46, (2003) (PubMed).

Salomon, Netzer, Wildbaum, Schif-Zuck, Maor, Karin: "Targeting the function of IFN-gamma-inducible protein 10 suppresses ongoing adjuvant arthritis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 5, pp. 2685-93, (2002) (PubMed).

Dragun, Lukitsch, Tullius, Qun, Park, Schneider, Luft, Haller: "Inhibition of intercellular adhesion molecule-1 with antisense deoxynucleotides prolongs renal isograft survival in the rat." in: Kidney international, Vol. 54, Issue 6, pp. 2113-22, (1999) (PubMed).

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4 (CD4 Produkte)

Synonyme: cd4 antikoerper, L3T4 antikoerper, Ly-4 antikoerper, CD4mut antikoerper, W3/25 antikoerper, p55 antikoerper, CD4 molecule antikoerper, carcinoembryonic antigen-related cell adhesion molecule 5 antikoerper, T-cell surface glycoprotein CD4 antikoerper, CD4 antigen antikoerper, Cd4 molecule antikoerper, CD4 antikoerper, LOC100125525 antikoerper, cd4 antikoerper, Cd4 antikoerper

Hintergrund: CD4 Molecule,CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).,T4/Leu-3, L3T4

Gen-ID: 24932

UniProt: P05540

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events