Polyglutamate Chain Antikörper

Produktnummer ABIN2745560

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Polyglutamate Chain in ICC, IHC und WB. Es zeigt Reaktivität gegenüber Proben von Diverse Spezies. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für Polyglutamate Chain Antikörper (ABIN2745560)

Target: Polyglutamate Chain

Reaktivität: Diverse Spezies

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Verwendungszweck: anti-Polyglutamate chain (polyE), pAb (IN105)

Spezifität: Recognizes C-terminally located linear alpha-glutamate chains of 4 and more glutamate residues.

Produktmerkmale: Polyclonal Antibody. Recognizes C-terminally located linear glutamate chains of 4 and more glutamate residues. Source: Rabbit. Applications: ICC, IHC, WB. Liquid. In PBS containing 0.02 % sodium azide. Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of alpha- and beta-tubulin. Two different mechanisms can generate microtubule diversity: the expression of different alpha- and beta-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on alpha- and beta-tubulin. Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation. These PTMs might have evolved to specifically regulate tubulin and microtubule functions. Polyglutamylation is a PTM that occurs when secondary glutamate side chains are formed on gamma-carboxyl groups of glutamate residues in a protein. Enzymes catalyzing polyglutamylation belong to the TTL-like (TTLL, Tubulin tyrosine ligase-like) family of glutamylases. Deglutamylases, the enzymes that reverse polyglutamylation, were identified within a novel family of CCPs (cytosolic carboxypeptidase). Subtle differences in polyglutamylation can be seen on diverse microtubules in different cell types. The functions of these modifications remain to be studied. However, its wide distribution strengthens the idea that it could be involved in fine-tuning a range of microtubule functions.

Immunogen: Polyglutamate peptide.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen

MSaad, Bewersdorf: "Light microscopy of proteins in their ultrastructural context." in: Nature communications, Vol. 11, Issue 1, pp. 3850, (2020) (PubMed).

Tosetti, Dos Santos Pacheco, Bertiaux, Maco, Bournonville, Hamel, Guichard, Soldati-Favre: "Essential function of the alveolin network in the subpellicular microtubules and conoid assembly in Toxoplasma gondii." in: eLife, Vol. 9, (2020) (PubMed).

Le Guennec, Klena, Gambarotto, Laporte, Tassin, van den Hoek, Erdmann, Schaffer, Kovacik, Borgers, Goldie, Stahlberg, Bornens, Azimzadeh, Engel, Hamel, Guichard: "A helical inner scaffold provides a structural basis for centriole cohesion." in: Science advances, Vol. 6, Issue 7, pp. eaaz4137, (2020) (PubMed).

Gambarotto, Zwettler, Le Guennec, Schmidt-Cernohorska, Fortun, Borgers, Heine, Schloetel, Reuss, Unser, Boyden, Sauer, Hamel, Guichard: "Imaging cellular ultrastructures using expansion microscopy (U-ExM)." in: Nature methods, Vol. 16, Issue 1, pp. 71-74, (2019) (PubMed).

Antigendetails

Target: Polyglutamate Chain

Hintergrund: Optional[Alternate Names/Synonyms]: Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of alpha- and beta-tubulin. Two different mechanisms can generate microtubule diversity: the expression of different alpha- and beta-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on alpha- and beta-tubulin. Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation. These PTMs might have evolved to specifically regulate tubulin and microtubule functions. Polyglutamylation is a PTM that occurs when secondary glutamate side chains are formed on gamma-carboxyl groups of glutamate residues in a protein. Enzymes catalyzing polyglutamylation belong to the TTL-like (TTLL, Tubulin tyrosine ligase-like) family of glutamylases. Deglutamylases, the enzymes that reverse polyglutamylation, were identified within a novel family of CCPs (cytosolic carboxypeptidase). Subtle differences in polyglutamylation can be seen on diverse microtubules in different cell types. The functions of these modifications remain to be studied. However, its wide distribution strengthens the idea that it could be involved in fine-tuning a range of microtubule functions. PolyE labels centrioles as they mature, such that two foci are present throughout the cell cycle.