alpha Actinin 4 Antikörper (C-Term, N-Term)

Produktnummer ABIN2745506

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch alpha Actinin 4 in WB, IHC und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schwein und Hamster.

Kurzübersicht für alpha Actinin 4 Antikörper (C-Term, N-Term) (ABIN2745506)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reaktivität: Human, Maus, Ratte, Schwein, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha Actinin 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails anti-alpha Actinin 4 Antikörper

Bindungsspezifität: AA 27-38, C-Term, N-Term

Spezifität: Recognizes human, mouse, rat, pig and Chinese hamster actinin-4.

Kreuzreaktivität: Hamster, Human, Maus, Schwein, Ratte (Rattus)

Kreuzreaktivität (Details): Does not cross-react with actinin-1, -2 or -3.

Aufreinigung: Antigen affinity purified.

Bestandteile: Contains Positive Control: Human platelet protein (Prod. No. AG-35T-0001), 500 μg supplied at 5mg/ml in SDS sample buffer. Use 5μl (25μg) per lane for Western blotting.

Immunogen: Synthetic peptide corresponding to aa 27-38 of the N-terminal alpha-Actinin-4, coupled via a C-terminal cysteine residue to KLH.

Anwendungsinformationen

Applikationshinweise: Use 5 μL (25 μg) per lane for Western blotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 1 mg/mL BSA and 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Details zu alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Andere Bezeichnung: alpha-Actinin-4

Hintergrund: Alpha-Actinin 4 is an actin-bundling protein of ~100 kDa that is associated with cell motility, endocytosis and cancer invasion. The alpha-actinin family comprises two non-muscle isoforms (alpha-actinin-1 and -4) and two skeletal muscle isoforms (alpha-actinin-2 and -3), with alpha-actinin-2 being also expressed in cardiac muscle. While alpha-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the alpha-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the alpha-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. alpha-Actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, alpha-actinin-4 forms a ternary complex with Ca2+/Calmodulin-dependent protein kinase II and densin-180, a protein of postsynaptic densities in CNS neurons. Ca2+-dependent association of alpha-actinin-4 with E3KARP is required for Ca2+-dependent inhibition of the Na+/H+ exchanger 3 (NHE3).

UniProt: O43707

Pathways: Proton Transport