CD34 Antikörper (N-Term)

Produktnummer ABIN2739286

Dieses Anti-CD34-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD34 in FACS, WB, IHC, IF, IHC (p) und IP. Geeignet für Human, Cynomolgus und Rhesusaffen.

Kurzübersicht für CD34 Antikörper (N-Term) (ABIN2739286)

Target: CD34

Reaktivität: Human, Cynomolgus, Rhesusaffen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Klon: QBEnd-10

Produktdetails anti-CD34 Antikörper

Bindungsspezifität: N-Term

Spezifität: Human CD34

Keine Kreuzreaktivität: Rind (Kuh), Hund, Ratte (Rattus), Schaf

Aufreinigung: Protein G purified

Immunogen: A detergent solubilized vesicular suspension prepared from human term placenta.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.25 - 0.5 μg/mL)

Usage: This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120  kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85 % of angiosarcoma and Kaposi's sarcoma, but with a lower specificity.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2°C to 8°C.

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Hintergrund: Name/Gene ID: CD34

Synonyms: CD34, CD34 Molecule, CD34 antigen

Gen-ID: 947