Actin Antikörper (N-Term)

Produktnummer ABIN2738124

Der Maus Monoklonal Anti-Actin-Antikörper wurde für WB, IHC, ELISA, IF, IHC (p), IP und FACS validiert. Er ist geeignet, Actin in Proben von Human, Ratte, Maus, Huhn, Kaninchen, Hund, Rind (Kuh), Affe, Schwein, Meerschweinchen, Cat, Pavian, Schaf und Ziege zu detektieren.

Kurzübersicht für Actin Antikörper (N-Term) (ABIN2738124)

Target: Actin

Reaktivität: Human, Ratte, Maus, Huhn, Kaninchen, Hund, Rind (Kuh), Affe, Schwein, Meerschweinchen, Cat, Pavian, Schaf, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Flow Cytometry (FACS)

Klon: 1A4

Produktdetails anti-Actin Antikörper

Bindungsspezifität: N-Term

Spezifität: Human Actin

Aufreinigung: Protein G purified

Immunogen: An N-Terminal decapeptide of the alpha smooth muscle isoform.

Type of Immunogen: Synthetic peptide

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle. however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin. No special pretreatment is required for the immunohistochemical staining of FFPE tissue. ELISA: Order BSA-free format.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 1 month or -20°C for longer. Avoid freeze/thaw cycles.

Details zu Actin

Target: Actin