MED1 Antikörper

Produktnummer ABIN272236

Produktdetails anti-MED1 Antikörper

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of TRAP220 protein. (region surrounding Pro697)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Andere Bezeichnung: MED1 (MED1 Produkte)

Synonyme: CRSP1 antikoerper, CRSP200 antikoerper, DRIP205 antikoerper, DRIP230 antikoerper, PBP antikoerper, PPARBP antikoerper, PPARGBP antikoerper, RB18A antikoerper, TRAP220 antikoerper, TRIP2 antikoerper, CRSP-1 antikoerper, MED1 antikoerper, pbp antikoerper, nbr1 antikoerper, crsp1 antikoerper, rb18a antikoerper, trip2 antikoerper, pparbp antikoerper, crsp200 antikoerper, drip205 antikoerper, drip230 antikoerper, ppargbp antikoerper, trap220 antikoerper, CG7162 antikoerper, Dmel\\CG7162 antikoerper, Med1 antikoerper, Med1/SOP3/TRAP220 antikoerper, Trap220 antikoerper, dTRAP220 antikoerper, AI480703 antikoerper, CRSP210 antikoerper, Pparbp antikoerper, l11Jus15 antikoerper, RGD1559552 antikoerper, mediator complex subunit 1 antikoerper, calcitonin-related polypeptide beta antikoerper, Mediator complex subunit 1 antikoerper, mediator of RNA polymerase II transcription subunit 1 antikoerper, MED1 antikoerper, CALCB antikoerper, med1 antikoerper, Med1 antikoerper

Hintergrund: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size from 20 to 240 kDa, including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, that are characterized by the presence of a nuclear receptor recognition motif which mediates the liganddependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes.Synonyms: ARC205, Activator-recruited cofactor 205 kDa component, CRSP1, CRSP200, DRIP205, DRIP230, Mediator complex subunit 1, PBP, PPARBP, PPARGBP, Peroxisome proliferator-activated receptor-binding protein, RB18A, TRAP220, TRIP2, Thyroid hormone receptor-associated protein complex 220 kDa component, Thyroid receptor-interacting protein 2, Vitamin D receptor-interacting protein complex component DRIP205, p53 regulatory protein RB18A

Molekulargewicht: approx. 170 kDa

Gen-ID: 5469

NCBI Accession: NP_004765

UniProt: Q15648

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha