NCBP2 Antikörper

Produktnummer ABIN272203

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NCBP2 in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für NCBP2 Antikörper (ABIN272203)

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCBP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NCBP2 Antikörper

Spezifität: This antibody detects endogenous levels of CBP20 protein. (region surrounding Arg21)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu NCBP2

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Andere Bezeichnung: NCBP2

Hintergrund: In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex (CBC). CBC consists of two cap binding proteins, CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80. CBP also plays a role in nonsense-mediated decay (NMD), a process which eliminates mRNAs, and prematurely terminates translation. CBP80-bound mRNA undergoes a pioneer round of translation before CBP80-CBP20 are replaced by eIF4E, and Upf2 and Upf3 proteins.Synonyms: CBP20, Nuclear cap-binding protein subunit 2, PIG55

Molekulargewicht: approx. 18 kDa

Gen-ID: 22916

NCBI Accession: NP_001036005

UniProt: P52298

Pathways: Ribonucleoprotein Complex Subunit Organization, Methionine Biosynthetic Process