NCBP2 Antikörper

Produktnummer ABIN272203

Produktdetails anti-NCBP2 Antikörper

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCBP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of CBP20 protein. (region surrounding Arg21)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu NCBP2

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Andere Bezeichnung: NCBP2 (NCBP2 Produkte)

Synonyme: CBP20 antikoerper, CG12357 antikoerper, Dmel\\CG12357 antikoerper, 20kDa antikoerper, 5930413E18Rik antikoerper, AA536802 antikoerper, AI843301 antikoerper, C79367 antikoerper, CHUNP6917 antikoerper, zgc:92592 antikoerper, CBC2 antikoerper, NIP1 antikoerper, ATCBP20 antikoerper, CAP-binding protein 20 antikoerper, MLN1.12 antikoerper, MLN1_12 antikoerper, Ncbp2 antikoerper, cap binding protein 20 antikoerper, nuclear cap binding protein subunit 2 antikoerper, nuclear cap binding protein subunit 2, 20kDa antikoerper, nuclear cap binding protein subunit 2 L homeolog antikoerper, nuclear cap-binding protein subunit 2 antikoerper, CAP-binding protein 20 antikoerper, Nuclear cap-binding protein subunit 2 antikoerper, Cbp20 antikoerper, Ncbp2 antikoerper, ncbp2 antikoerper, NCBP2 antikoerper, ncbp2.L antikoerper, CBP20 antikoerper, ncbp-2 antikoerper

Hintergrund: In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex (CBC). CBC consists of two cap binding proteins, CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80. CBP also plays a role in nonsense-mediated decay (NMD), a process which eliminates mRNAs, and prematurely terminates translation. CBP80-bound mRNA undergoes a pioneer round of translation before CBP80-CBP20 are replaced by eIF4E, and Upf2 and Upf3 proteins.Synonyms: CBP20, Nuclear cap-binding protein subunit 2, PIG55

Molekulargewicht: approx. 18 kDa

Gen-ID: 22916

NCBI Accession: NP_001036005

UniProt: P52298

Pathways: Ribonucleoprotein Complex Subunit Organization, Methionine Biosynthetic Process