CBX5 Antikörper

Produktnummer ABIN272193

Der Kaninchen Polyklonal anti-CBX5 Antikörper (ABIN272193) detektiert spezifisch CBX5 in WB, IF und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für CBX5 Antikörper (ABIN272193)

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CBX5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CBX5 Antikörper

Spezifität: This antibody detects endogenous levels of HP1-alpha/CBX5 protein. (region surrounding Lys69)

Kreuzreaktivität (Details): Species reactivity (tested):Human and Mouse.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10000-1/20000. Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CBX5

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Andere Bezeichnung: CBX5

Hintergrund: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1), HP1α, HP1β and HP1γ. As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.Synonyms: Chromobox protein homolog 5, HP1 alpha, HP1-alpha, HP1A, Heterochromatin protein 1 homolog alpha, p25

Molekulargewicht: approx. 22 kDa

Gen-ID: 23468

NCBI Accession: NP_001120793

UniProt: P45973