AIRE Antikörper

Produktnummer ABIN272137

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch AIRE in WB und IF. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für AIRE Antikörper (ABIN272137)

Target: AIRE (Autoimmune Regulator (AIRE))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AIRE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-AIRE Antikörper

Spezifität: This antibody detects endogenous levels of AIRE-1 protein. (region surrounding Pro126)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Andere Bezeichnung: AIRE / APECED

Hintergrund: The autoimmune regulator gene, which is defective in the hereditary autoimmune disease APECED, encodes the transcriptional activator AIRE. AIRE is expressed in the medullary epithelial cells and monocyte-dendritic cells of the thymus, with lower expression in the spleen, fetal liver and lymph nodes. In adult tissue, AIRE expression in the thymus is confined to the medulla and the cortico-medullary junction, where it is modulated by thymocytes undergoing negative selection. At the cellular level, AIRE is located in microtubular structures of the cytoskeleton and in discrete nuclear dots resembling ND10 nuclear bodies. AIRE is induced by developing early thymocytes and is associated with the correct establishment of a regular thymic environment. AIRE regulates thymic architecture via transcriptional control of downstream target genes. AIRE mutations in APECED patients may affect thymic T cell selection and the formation of self-tolerance.Synonyms: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator

Molekulargewicht: approx. 60 kDa

Gen-ID: 326

NCBI Accession: NP_000374

UniProt: O43918

Pathways: Chromatin Binding