CLOCK Antikörper

Produktnummer ABIN272135

Der Kaninchen Polyklonal anti-CLOCK Antikörper wird verwendet zum Nachweis von CLOCK in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für CLOCK Antikörper (ABIN272135)

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CLOCK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CLOCK Antikörper

Spezifität: This antibody detects endogenous levels of Clock protein. (region surrounding Glu270)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CLOCK

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Andere Bezeichnung: CLOCK

Hintergrund: Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. Clock, a member of the basic-helix-loop-helix-PAS (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the Clock gene have been shown to increase the length of the endogenous period and to cause a loss of rhythmicity of circadian oscillations. Clock contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation capabilities. It has been speculated that Clock may regulate circadian rhythmicity in combination with other proteins such as Per. Per is also a PAS-domain containing protein that exhibits circadian function. Highest expression of Clock is seen in the hypothalamus and the eye.Synonyms: BHLHE8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, KIAA0334

Molekulargewicht: approx. 95 kDa

Gen-ID: 9575

NCBI Accession: NP_004889

UniProt: O15516

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism