PBK Antikörper

Produktnummer ABIN272056

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch PBK in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für PBK Antikörper (ABIN272056)

Target: PBK (PDZ Binding Kinase (PBK))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PBK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-PBK Antikörper

Spezifität: This antibody detects endogenous levels of PBK/TOPK protein. (region surrounding Ile4)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 21000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should bedetermined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu PBK

Target: PBK (PDZ Binding Kinase (PBK))

Andere Bezeichnung: PBK / TOPK

Hintergrund: PBK (PDZ binding kinase) belongs to the serine/threonine protein kinase family. It is related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family and phosphorylates MAP kinase p38. It is active only in mitosis. When phosphorylated it forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. It makes a kinase-substrate complex with cdk1/cyclin B1 and PRC1 on microtubules during mitosis, enhances the cdk1/cyclin B1-dependent phosphorylation of PRC1 and thereby strongly promotes cytokinesis. It may play a role in the activation of lymphoid cells.Synonyms: CT84, Cancer/testis antigen 84, Lymphokine-activated killer T-cell-originated protein kinase, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, SPK, T-LAK cell-originated protein kinase

Molekulargewicht: approx. 36 kDa

Gen-ID: 55872

NCBI Accession: NP_060962

UniProt: Q96KB5