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Actin Antikörper (pan)

Name: Actin Antikörper (pan)
SKU: ABIN271986
Price: 891.99 EUR
Availability: InStock

Reaktivität: Human, Ratte, Maus WB, IHC (p), IF Wirt: Kaninchen Polyclonal unconjugated

Image no. 2 for anti-Actin (pan) antibody (ABIN271986) (Actin Antikörper (pan))

Image no. 1 for anti-Actin (pan) antibody (ABIN271986) (Actin Antikörper (pan))

2 Abbildungen

Produktnummer ABIN271986

Datenblatt (PDF)

Target Alle Actin Antikörper anzeigen

Actin
Bindungsspezifität
16

15

13

12

11

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
pan
Reaktivität
98

78

74

24

22

21

12

11

6

5

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Human, Ratte, Maus
Wirt
83

41

3

1

1
Kaninchen
Passende Sekundärantikörper
Klonalität
76

53
Polyklonal
Konjugat
73

6

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
Dieser Actin Antikörper ist unkonjugiert
Applikation
101

36

30

29

28

26

24

18

16

15

10

3

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Spezifität

This antibody detects endogenous levels ofActin-pan (α/β/γ) protein (region surrounding Gln49)

Kreuzreaktivität (Details)

Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Aufreinigung

Affinity-chromatography

Reinheit

> 95 % (by SDS-PAGE)

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anti-Actin (C-Term) antibody
Reaktivität: Human, Ratte, Maus WB, IHC, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-Actin antibody
Reaktivität: Human, Ratte, Maus, Huhn, Hund, Rind (Kuh), Schwein, Meerschweinchen WB, ELISA, IHC (p), IF (cc), IF (p), FACS Wirt: Kaninchen Polyclonal unconjugated

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Reaktivität: Oryza sativa, Gerste, Arabidopsis, Nicotiana tabacum WB Wirt: Kaninchen Polyclonal unconjugated

anti-Actin (AA 367-377) antibody
Reaktivität: Human, Ratte WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Actin (AA 21-70) antibody
Reaktivität: Human, Ratte, Maus WB, IHC, ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Actin (AA 189-218) antibody
Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Actin (AA 38-67), (N-Term) antibody
Reaktivität: Human, Maus WB, IHC (p), IF Wirt: Kaninchen Polyclonal unconjugated

anti-Actin antibody
Reaktivität: Human, Ratte, Maus, Huhn, Hund WB, IF Wirt: Maus Monoclonal C4-actin unconjugated

Applikationshinweise

ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1,0 mg/mL

Buffer

PBS, pH 7.2, 0.05 % sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Avoid repeated freezing and thawing.

Lagerung

4 °C/-20 °C

Informationen zur Lagerung

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

Actin

Abstract

Actin Produkte

Hintergrund

Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90 % ".

Molekulargewicht

approx. 45 kDa