Actin Antikörper (pan)

Produktnummer ABIN271986

Dieses Anti-Actin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Actin in WB, IF und IHC (p). Geeignet für Human, Ratte und Maus.

Kurzübersicht für Actin Antikörper (pan) (ABIN271986)

Target: Actin

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Actin Antikörper

Bindungsspezifität: pan

Spezifität: This antibody detects endogenous levels ofActin-pan (α/β/γ) protein (region surrounding Gln49)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Aufreinigung: Affinity-chromatography

Reinheit: > 95 % (by SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: PBS, pH 7.2, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Actin

Target: Actin

Hintergrund: Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90 % ".

Molekulargewicht: approx. 45 kDa