NCK2 Antikörper

Produktnummer ABIN271941

Der Kaninchen Polyklonal anti-NCK2 Antikörper (ABIN271941) detektiert spezifisch NCK2 in IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für NCK2 Antikörper (ABIN271941)

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCK2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NCK2 Antikörper

Spezifität: This antibody detects endogenous levels of NCK2 protein. (region surrounding Ile364)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 1000approx. 1: 5000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu NCK2

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Andere Bezeichnung: NCK2

Hintergrund: The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKα) and NCK2 (NCKβ), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of NCK1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009), overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2.The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, respectively.Synonyms: NCK adaptor protein 2, SH2/SH3 adaptor protein NCK-beta

Molekulargewicht: approx. 47 kDa

Gen-ID: 8440

NCBI Accession: NP_001004720

UniProt: O43639

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization