Integrin beta 3 Antikörper

Produktnummer ABIN7687678

Der Maus Monoklonal anti-Integrin beta 3 Antikörper (Klon PM6-13) (ABIN7687678) detektiert spezifisch Integrin beta 3 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Integrin beta 3 Antikörper (ABIN7687678)

Target: Integrin beta 3 (ITGB3)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: PM6-13

Produktdetails anti-Integrin beta 3 Antikörper

Verwendungszweck: Mouse monoclonal antibody to CD61

Spezifität: Recognizes human CD61

Produktmerkmale: Mouse monoclonal antibody to CD61

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD61.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Andere Bezeichnung: CD61

Hintergrund: GP3A, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa, CD antigen CD61

Gen-ID: 3690

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex