ITGA4 Antikörper

Produktnummer ABIN7687666

Der Maus Monoklonal anti-ITGA4 Antikörper (Klon BU49) (ABIN7687666) detektiert spezifisch ITGA4 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für ITGA4 Antikörper (ABIN7687666)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGA4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: BU49

Produktdetails anti-ITGA4 Antikörper

Verwendungszweck: Mouse monoclonal antibody to CD49d

Spezifität: Recognizes human CD49d

Produktmerkmale: Mouse monoclonal antibody to CD49d

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD49d.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Andere Bezeichnung: CD49d

Hintergrund: CD49D, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha, CD antigen CD49d

Gen-ID: 3676

UniProt: P13612

Pathways: Integrin Complex