T Cell Receptor (TCR) V beta 12 Antikörper (Biotin)

Produktnummer ABIN2689952

Dieser Maus Monoklonal Antikörper detektiert spezifisch T Cell Receptor (TCR) V beta 12 in FACS. Es zeigt Reaktivität gegenüber Proben von Maus. Es wurde in 3+ Publikationen zitiert.

Kurzübersicht für T Cell Receptor (TCR) V beta 12 Antikörper (Biotin) (ABIN2689952)

Target: T Cell Receptor (TCR) V beta 12

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Flow Cytometry (FACS)

Klon: MR11

Produktdetails

Produktmerkmale: The MR11-1 antibody reacts with the Vβ 12 T-cell Receptor (TCR) of mice having the b haplotype (e.g., C57BL, C58, DBA/1) of the Tcrb gene complex1 The Tcrb-V12 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 12 TCR-bearing T lymphocytes are clonally eliminated in mice expressing I-E and superantigens encoded by Mtv-8 (Mlsf, Dvb11.1), Mtv-9 (Etc-1, Mlsf, Dvb11.2) and/or Mtv-11 (Mlsf, Dvb11.3) proviruses (eg, A, AKR, BALB/c, CBA/J, C3H, DBA/2). Activation of Vβ 12 TCR-expressing T cells by these determinants is dependent upon presentation by I-E. C57BL/6 spleen T cells expressing Vβ 12 TCR are among the predominant responders to MAIDS virus superantigen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of Vß12 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated MR11-1, PE-conjugated anti-mouse CD4 RM4-5 (Cat. No. 553048/553049), and PE-conjugated anti-mouse CD8a 53-6.7 (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC, Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-Mouse Vβ 12 T-Cell Receptor - Biotin - Clone MR11-1 - Isotype Mouse IgG1, κ - Reactivity Ms - 0.25 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Not Reported

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen

Vacchio, Hodes: "Selective decreases in T cell receptor V beta expression. Decreased expression of specific V beta families is associated with expression of multiple MHC and non-MHC gene products." in: The Journal of experimental medicine, Vol. 170, Issue 4, pp. 1335-46, (1989) (PubMed).

Haqqi, Banerjee, Anderson, David: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." in: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, (1989) (PubMed).

Behlke, Chou, Huppi, Loh: "Murine T-cell receptor mutants with deletions of beta-chain variable region genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 767-71, (1986) (PubMed).

Antigendetails

Target: T Cell Receptor (TCR) V beta 12

Andere Bezeichnung: Vbeta 12 T-Cell Receptor