SOX1 Antikörper

Produktnummer ABIN2689905

Produktdetails anti-SOX1 Antikörper

Target: SOX1 (SRY (Sex Determining Region Y)-Box 1 (SOX1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), BioImaging (BI)

Marke: BD Pharmingen™

Produktmerkmale: The N23-844 monoclonal antibody reacts with human Sox1, a member of the SOX [SRY (sex determining region Y)-HMG-box] family of transcription factors. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. It is one of the earliest transcription factors to be expressed in ectodermal cells committed to the neural fate. Sox1 is expressed in both embryonic and somatic neural stem and progenitor cells, and it is down regulated during neuronal differentiation in many neuronal subtypes. Western Blot analysis of SOX1. Lysate from neural stem cells (NSCs) derived from H9 human ES cells (WiCell, Madison, WI) was probed with Purified Mouse anti-Human SOX1 monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 μg/mL (lane 3). SOX1 is identified as a tight doublet of almost 39 kDa. Immunofluorescent staining of Human SOX1. NSCs derived from H9 human ES cells (WiCell Madison, WI) were fixed, permeabilized with BD Perm/Wash™ Buffer (Cat No. 554723), and stained with purified anti-human SOX1 (0.5 μg/test). The second step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies) and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. Triton™ X-100 is also suitable for permeabilization.

BD Pharmingen™ Purified Mouse anti-Human SOX1 - Purified - Clone N23-844 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Sox1 Peptide

Klon: N23

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-SOX1 Antikörper (ABIN2689905)

Kan, Israsena, Zhang, Hu, Zhao, Jalali, Sahni, Kessler: "Sox1 acts through multiple independent pathways to promote neurogenesis." in: Developmental biology, Vol. 269, Issue 2, pp. 580-94, (2004) (PubMed).

Wilson, Koopman: "Matching SOX: partner proteins and co-factors of the SOX family of transcriptional regulators." in: Current opinion in genetics & development, Vol. 12, Issue 4, pp. 441-6, (2002) (PubMed).

Pevny, Sockanathan, Placzek, Lovell-Badge: "A role for SOX1 in neural determination." in: Development (Cambridge, England), Vol. 125, Issue 10, pp. 1967-78, (1998) (PubMed).

Malas, Duthie, Mohri, Lovell-Badge, Episkopou: "Cloning and mapping of the human SOX1: a highly conserved gene expressed in the developing brain." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 8, Issue 11, pp. 866-8, (1998) (PubMed).

Details zu SOX1

Target: SOX1 (SRY (Sex Determining Region Y)-Box 1 (SOX1))

Andere Bezeichnung: SOX1 (SOX1 Produkte)

Synonyme: BB176347 antikoerper, Sox-1 antikoerper, XlSox1 antikoerper, xSox1 antikoerper, wu:fi38c08 antikoerper, wu:fj38h03 antikoerper, zgc:92865 antikoerper, SRY-box 1 antikoerper, SRY (sex determining region Y)-box 1 antikoerper, SRY-box 1 S homeolog antikoerper, SRY (sex determining region Y)-box 1b antikoerper, SRY box 1 antikoerper, SRY (sex determining region Y)-box 1a antikoerper, SOX1 antikoerper, Sox1 antikoerper, sox1.S antikoerper, sox1b antikoerper, sox1a antikoerper

Molekulargewicht: 39 kDa