CCL5 Antikörper (Chemokine (C-C Motif) Ligand 5)

Details for Product anti-CCL5 Antibody No. ABIN2689891, Anbieter: Anmelden zum Anzeigen
Antigen
  • Rantes
  • Scya5
  • D17S136E
  • RANTES
  • SCYA5
  • SIS-delta
  • SISd
  • TCP228
  • eoCP
  • MuRantes
  • C-C motif chemokine 5-like
  • C-C motif chemokine ligand 5
  • chemokine (C-C motif) ligand 5
  • LOC100230935
  • CCL5
  • Ccl5
Reaktivität
Human
199
107
50
25
14
4
4
3
3
3
1
1
1
1
1
Wirt
Maus
192
83
37
5
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CCL5 Antikörper ist unkonjugiert
55
24
14
11
8
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC)
224
183
75
55
38
32
18
15
14
13
7
5
3
3
3
2
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Marke BD Pharmingen™
Immunogen Recombinant human RANTES protein
Klon 2D5
Isotyp IgG1, kappa
Produktmerkmale The 2D5 antibody reacts with human Regulated upon Activation, Normal T cells Expressed and Secreted (RANTES). The immunogen used to generated this hybridoma was recombinant human RANTES protein. Immunocytochemical staining for RANTES. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37 °C with LPS (Sigma Cat. No. L-8274, 1 μg / ml) in the presence of BD GolgiStop™ (Cat. No. 554724). The activated cells were harvested and stained by immunocytochemistry using a three-step staining procedure employing a Biotin Goat Anti-Mouse Ig secondary antibody and a Horseradish Peroxidase (HRP) based detection system (Nomarski optics, original magnification 400X).

BD Pharmingen™ Purified Mouse Anti-Human RANTES - Purified - Clone 2D5 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.25 mg
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung RANTES (CCL5 Antibody Abstract)
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C.
Produkt verwendet in: Sticherling, Küpper, Koltrowitz, Bornscheuer, Kulke, Klinger, Wilhelm, Kameyoshi, Christophers, Schröder: "Detection of the chemokine RANTES in cytokine-stimulated human dermal fibroblasts." in: The Journal of investigative dermatology, Vol. 105, Issue 4, pp. 585-91, 1995 (PubMed).

Hsu, Raine, Fanger: "Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 29, Issue 4, pp. 577-80, 1981 (PubMed).

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