Pan Carcinoma Antikörper

Produktnummer ABIN2689873

Zitiert in 4+ Publikationen. Der Maus Monoklonal anti-Pan Carcinoma Antikörper (Klon KS1-4) (ABIN2689873) detektiert spezifisch Pan Carcinoma in IHC (fro), FACS, IF und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Pan Carcinoma Antikörper (ABIN2689873)

Target: Pan Carcinoma

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: KS1-4

Produktdetails

Produktmerkmale: KS1/4 antigen (Ag), defined by the KS1/4 antibody (KS1/4), is expressed in many epithelial-derived carcinomas and normal epithelial cell surfaces. The cDNA encoding KS1/4 has been isolated and contains an open reading frame of 314 amino acids including a putative signal sequence. KS1/4 Ag migrates as three glycosylated polypeptides with molecular masses of 35, 40 and 42 kDa. The 40 and 42 kDa species are similar proteins and appear to differ only by their degree of N-linked glycosylation. The 35 kDa species results from proteolytic cleavages of the larger molecular weight proteins. KS1/4 was first described as a monoclonal antibody which recognized a lung adenocarcinoma associated antigen. In oncolytic drug targeting studies, KS1/4 suppressed the growth of human lung tumor xenografts in athymic nude mice. Subsequent studies showed that KS1/4 reacts with a variety of tumor tissues including colon, breast, ovarian, and pancreas. It also recognizes normal epithelium including colon, stomach, small intestine, liver, kidney, lung, pancreas, skin and ovary. These results suggest that KS1/4 Ag represents an epithelial cell/epithelial-derived carcinoma marker. KS1/4 recognizes a 40 - 42 kDa antigen expressed on the cell surfaces of a variety of epithelial tumors and normal epithelial cell types. KS1/4 also recognizes a 35 kDa proteolytic fragment. UCLA-P3 cells derived from a human adenocarcinoma of the lung were used as immunogen. Profile of UCLA-P3 lung adenocarcinoma cells analyzed by flow cytometry. Cells were incubated with either KS1/4 (Cat. No. 554276) or an isotype control, followed by a FITC-conjugated second step antibody.

BD Pharmingen™ Purified Mouse Anti-40 kDa Pan Carcinoma - Purified - Clone KS1/4 - Isotype Mouse IgG2a - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: UCLA-P3 cells

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen

Perez, Walker: "Isolation and characterization of a cDNA encoding the KS1/4 epithelial carcinoma marker." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 10, pp. 3662-7, (1989) (PubMed).

Bumol, Marder, DeHerdt, Borowitz, Apelgren: "Characterization of the human tumor and normal tissue reactivity of the KS1/4 monoclonal antibody." in: Hybridoma, Vol. 7, Issue 4, pp. 407-15, (1988) (PubMed).

Spearman, Goodwin, Apelgren, Bumol: "Disposition of the monoclonal antibody-vinca alkaloid conjugate KS1/4-DAVLB (LY256787) and free 4-desacetylvinblastine in tumor-bearing nude mice." in: The Journal of pharmacology and experimental therapeutics, Vol. 241, Issue 2, pp. 695-703, (1987) (PubMed).

Varki, Reisfeld, Walker: "Antigens associated with a human lung adenocarcinoma defined by monoclonal antibodies." in: Cancer research, Vol. 44, Issue 2, pp. 681-7, (1984) (PubMed).

Antigendetails

Target: Pan Carcinoma