Nkx2-2 Antikörper

Produktnummer ABIN2689863

Produktdetails anti-Nkx2-2 Antikörper

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nkx2-2 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), BioImaging (BI), Intracellular Staining (ICS)

Marke: BD Pharmingen™

Produktmerkmale: The 74.5A5 monoclonal antibody specifically binds to Homeobox protein Nkx-2.2 (Nkx2.2), which is a transcription factor required for the differentiation of ventral neuronal populations in the central nervous system and endocrine cell populations in the pancreas and intestine. Within the pancreas, Nkx2.2 is expressed in many cell subsets and plays important roles in islet and β-cell differentiation and maturation. Mice with a homozygous disruption of the Nkx2-2 gene lack mature β cells and die shortly after birth with severe hyperglycemia. Similarly to the Nkx2.2-ablated mice, homozygous mutations in the human NKX2-2 gene have been described in diabetic patients and associated with the etiology of neonatal diabetes. The NKX2-2 gene is also a target of the Ewing sarcoma specific protein EWS-FLI. Nkx2.2 expression can be monitored in order to determine cell fate changes in cultures for the generation of human pluripotent stem cell-derived insulin-producing cells as well as oligodendrocyte progenitors. LEFT: Western blot analysis of Nkx2.2 expression in human embryonic kidney cell lysate. Lysates of 293-F cells (Life Technologies R790-07) were prepared for electrophoresis (SDS-PAGE) in a 2D Tris-Glycine polyacrylamide gel. The proteins were transferred to PVDF membranes and then probed with 0.5, 0.25, 0.125, 0.062 μg/mL (Lanes 1-4, respectively) of Purified Mouse Anti-Nkx2.2. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). RIGHT: Flow cytometric analysis of Nkx2.2 expression in human embryonic kidney cells. 293-F cells (Life Technologies R790-07) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574). The fixed cells were then stained with either Purified Mouse IgG2b, Isotype Control (Cat. No. 556654, dashed line) or Purified Mouse Anti-Nkx2.2 (solid line). Specific staining was detected with PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System. LEFT: Immunohistochemical staining Nkx2.2 in human pancreas. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG2b κ Isotype Control (Cat. No.556654, left) or Purified Mouse Anti-Nkx2.2 (right). A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification: 40x. RIGHT: Immunofluorescent staining of Nkx2.2 in mouse insulinoma cells. Beta-TC-6 cells (ATCC CRL-11506) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050), and stained with Purified Mouse Anti-Nkx2.2 at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 Goat Anti-Mouse Ig (Life Technologies, pseudo-colored green), and the counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software. Permeabilization with BD Perm/Wash™ Buffer (Cat No. 554723) or 0.1 % Triton™ X-100 buffer is also suitable for use with this antibody. 564731 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified Mouse Anti-Nkx2.2 - Purified - Clone 74.5A5 - Isotype Mouse IgG2b, κ - Reactivity Hu, Ms, Chick, Rat - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Chicken Nkx2.2 Recombinant Protein

Klon: 74-5A5

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-Nkx2-2 Antikörper (ABIN2689863)

Yoshida, Sekine, Tsuta, Fukayama, Furuta, Tsuda: "NKX2.2 is a useful immunohistochemical marker for Ewing sarcoma." in: The American journal of surgical pathology, Vol. 36, Issue 7, pp. 993-9, (2012) (PubMed).

DAmour, Bang, Eliazer, Kelly, Agulnick, Smart, Moorman, Kroon, Carpenter, Baetge: "Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells." in: Nature biotechnology, Vol. 24, Issue 11, pp. 1392-401, (2006) (PubMed).

Briscoe, Sussel, Serup, Hartigan-OConnor, Jessell, Rubenstein, Ericson: "Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling." in: Nature, Vol. 398, Issue 6728, pp. 622-7, (1999) (PubMed).

Sussel, Kalamaras, Hartigan-OConnor, Meneses, Pedersen, Rubenstein, German: "Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells." in: Development (Cambridge, England), Vol. 125, Issue 12, pp. 2213-21, (1998) (PubMed).

Ericson, Rashbass, Schedl, Brenner-Morton, Kawakami, van Heyningen, Jessell, Briscoe: "Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling." in: Cell, Vol. 90, Issue 1, pp. 169-80, (1997) (PubMed).

Details zu Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Andere Bezeichnung: Nkx2.2 (Nkx2-2 Produkte)

Hintergrund: Synonyms: NKX2-2, NKX2B, Nkx2-2, Nkx-2.2, NK-2 homolog B, glycoprotein GP330

Molekulargewicht: 30-40 kDa

Pathways: Dopaminergic Neurogenesis