Mast Cells Antikörper

Produktnummer ABIN2689832

Dieser Maus Monoklonal Antikörper detektiert spezifisch Mast Cells in FACS, IP, Sep und IHC. Es zeigt Reaktivität gegenüber Proben von Ratte. Es wurde in 3+ Publikationen zitiert.

Kurzübersicht für Mast Cells Antikörper (ABIN2689832)

Target: Mast Cells

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Separation (Sep), Immunohistochemistry (IHC)

Klon: AR32AA4

Produktdetails

Produktmerkmale: The AR32AA4 (AA4) antibody reacts with two distinct α-galactosyl derivatives of the ganglioside GD1b (disialogangliosides). The mAb AA4 reacts with four indistinct bands ranging in size from 56 - 110 kDa. Binding of the AA4 antibody to RBL-2H3 cells has been shown to inhibit IgE-mediated histamine release by inhibiting FcεR1 signal transduction events. The mAb AA4 has been shown to be useful for immunomagnetic mast cell separation from rat bone marrow and peritoneal lavage cell populations. This corresponds with reports that the AA4-binding epitope is expressed at higher levels than the FcεR1 on RBL-2H3 cells. Staining of mAb AR32AA4 on RBL (ATCC CRL-2256) cells. RBL cells were incubated with either Purified Mouse IgG1, κ isotype control (Cat. no. 557273, open dash line overlay) or purified AR32AA4 mAb (shaded histogram), followed by biotinylated F(ab')2 rat antimouse IgG and finally Streptavidin-FITC (Cat. no. 554060). The cells in both the shaded histogram and the overlay were gated on 7-AAD negative cells. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat Mast Cells - Purified - Clone AR32AA4 (also known as AA4) - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat basophilic leukemia cell line

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen

Jamur, Grodzki, Moreno, Swaim, Siraganian, Oliver: "Immunomagnetic isolation of rat bone marrow-derived and peritoneal mast cells." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 45, Issue 12, pp. 1715-22, (1998) (PubMed).

Stephan, Seibt, Dukanovic, Skasa, Swaim, Berenstein, Siraganian, Wahn: "Anti-ganglioside monoclonal antibody AA4 selectively inhibits IgE-induced signal transduction pathways in rat basophilic leukemia cells." in: Molecular immunology, Vol. 34, Issue 3, pp. 227-35, (1997) (PubMed).

Basciano, Berenstein, Kmak, Siraganian: "Monoclonal antibodies that inhibit IgE binding." in: The Journal of biological chemistry, Vol. 261, Issue 25, pp. 11823-31, (1986) (PubMed).

Antigendetails

Target: Mast Cells