Lgr5 Central LRR Antikörper

Produktnummer ABIN2689806

Es sind 7+ Publikationen für dieses Produkt verfügbar. Der Ratte Monoklonal anti-Lgr5 Central LRR Antikörper wird verwendet zum Nachweis von Lgr5 Central LRR in Proben von Human. Er wurde validiert für BI, FACS und IF.

Kurzübersicht für Lgr5 Central LRR Antikörper (ABIN2689806)

Target: Lgr5 Central LRR

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 4D11F8

Produktdetails

Kreuzreaktivität (Details): Mouse (Lack of Reactivity Confirmed in Development)

Produktmerkmale: Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) is a seven transmembrane-domain receptor that is a target gene for Wnt and marks stem cells in the small intestine, colon, stomach, and hair follicle. Lgr5 was initially identified as a potential stem cell marker due to restricted expression of Lgr5 in the intestinal crypt and labeling of rapidly cycling cells of the colon and intestine. Using both lineage tracing and organoid culture experiments, Lgr5 positive cells are capable of generating all types of the small intestine epithelium hence indicating that Lgr5 marks stem cells of the small intestine and colon. R-spondin growth factors, which are secreted agonists of the Wnt pathway, bind Lgr5. The binding of R-spondins to Lgr5 leads to recruitment of the Frizzled/LRP Wnt receptor complex, which binds to Wnt ligands and leads to downstream Wnt signaling. Lgr5 is up-regulated in colon and ovarian cancers and has been implicated in promotion of tumor growth and metastasis. The 4D11F8 monoclonal antibody recognizes an epitope in the center of the leucine-rich repeat (LRR) region of Human Lgr5. Flow cytometric analysis of human LGR5. Colorectal adenocarcinoma cells LS 174T (ATCC CL-188) were stained with either Purified Rat IgG2b isotype control (Cat. No. 553986) or Purified Rat anti-human LGR5 (Central LRR) monoclonal antibody (solid line) at matched concentrations. Cells were harvested with Accutase™ Cell Detachment Solution (Cat. No. 561527).The second step reagent was PE goat anti-Rat Ig (Cat. No. 550767). The histograms were derived from gated events based on light scattering characteristics of the LS 174T cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system. Immunoflourescent staining of human LGR5. Colorectal adenocarcinoma cells LS 174T transfected with human LGR5 (Cells from Dr. Hans Clevers, Hubrecht Institute) were fixed and stained with Purified Rat anti-human LGR5 (Central LRR) monoclonal antibody (pseudo colored green) at 2.5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-rat Ig (Life Technologies) and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. 562732 Rev. 2 Page 1 of 2

BD Pharmingen™ Purified Rat anti-Human Lgr5 (Central LRR) - Purified - Clone 4D11F8 (also known as 4D11) - Isotype Rat IgG2b, λ - Reactivity Hu, Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human LGR5 DNA

Isotyp: IgG2b lambda

Anwendungsinformationen

Applikationshinweise: Western blot, Immunohistochemistry-frozen, Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen

Yui, Nakamura, Sato, Nemoto, Mizutani, Zheng, Ichinose, Nagaishi, Okamoto, Tsuchiya, Clevers, Watanabe: "Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell." in: Nature medicine, Vol. 18, Issue 4, pp. 618-23, (2012) (PubMed).

Carmon, Gong, Lin, Thomas, Liu: "R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 28, pp. 11452-7, (2011) (PubMed).

Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, (2011) (PubMed).

de Lau, Barker, Low, Koo, Li, Teunissen, Kujala, Haegebarth, Peters, van de Wetering, Stange, van Es, Guardavaccaro, Schasfoort, Mohri, Nishimori, Mohammed, Heck, Clevers: "Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling." in: Nature, Vol. 476, Issue 7360, pp. 293-7, (2011) (PubMed).

Barker, Huch, Kujala, van de Wetering, Snippert, van Es, Sato, Stange, Begthel, van den Born, Danenberg, van den Brink, Korving, Abo, Peters, Wright, Poulsom, Clevers: "Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro." in: Cell stem cell, Vol. 6, Issue 1, pp. 25-36, (2010) (PubMed).

Jaks, Barker, Kasper, van Es, Snippert, Clevers, Toftgård: "Lgr5 marks cycling, yet long-lived, hair follicle stem cells." in: Nature genetics, Vol. 40, Issue 11, pp. 1291-9, (2008) (PubMed).

Barker, van Es, Kuipers, Kujala, van den Born, Cozijnsen, Haegebarth, Korving, Begthel, Peters, Clevers: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, (2007) (PubMed).

Antigendetails

Target: Lgr5 Central LRR

Hintergrund: Synonyms: GPR49, GPR67, HG38