Human Papilloma Virus 16 Capsid Protein (HPV-16 L1) Antikörper

Produktnummer ABIN2689560

Es sind 4+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-Human Papilloma Virus 16 Capsid Protein Antikörper wird verwendet zum Nachweis von Human Papilloma Virus 16 Capsid Protein in Proben von Human. Er wurde validiert für IHC (f) und IHC (fro).

Kurzübersicht für Human Papilloma Virus 16 Capsid Protein (HPV-16 L1) Antikörper (ABIN2689560)

Target: Human Papilloma Virus 16 Capsid Protein (HPV-16 L1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: CAMVIR

Produktdetails

Produktmerkmale: More than 60 different types of human papilloma viruses (HPVs) have been isolated. The human papilloma viruses encode three late proteins, which are produced only in terminally differentiating keratinocytes, two of which (the L1 and L2 proteins) are structural components of the virion. Virus-like particles can be assembled by over-expressing L1 and L2 in vitro, demonstrating that L1 and L2 are necessary and sufficient components of the HPV capsomeres. HPV-16 has been reported to migrate at a reduced molecular weight of ~57 kD by SDS-PAGE. The CAMVIR-1 antibody reacts with the L1 protein of HPV-16 and may also cross-react with other HPV types, such as HPV-33. Immunohistochemistry for HPV type 16. A formalin-fixed, paraffin-embedded section from human cervix infected with HPV type 16 was stained with the Purified Mouse Anti-HPV 16 L1 antibody. Cells expressing the L1 protein of the HPV 16 virus can be identifed by the intense brown labeling (magnification 20X).

BD Pharmingen™ Purified Mouse Anti-HPV-16 L1 - Purified - Clone CAMVIR-1 - Isotype Mouse IgG2a, κ - Reactivity Hu - 1 mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen

Doorbar, Ely, Coleman, Hibma, Davies, Crawford: "Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein." in: Virology, Vol. 187, Issue 1, pp. 353-9, (1992) (PubMed).

Zhou, Sun, Stenzel, Frazer: "Expression of vaccinia recombinant HPV 16 L1 and L2 ORF proteins in epithelial cells is sufficient for assembly of HPV virion-like particles." in: Virology, Vol. 185, Issue 1, pp. 251-7, (1991) (PubMed).

McLean, Churcher, Meinke, Smith, Higgins, Stanley, Minson: "Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus." in: Journal of clinical pathology, Vol. 43, Issue 6, pp. 488-92, (1990) (PubMed).

de Villiers: "Heterogeneity of the human papillomavirus group." in: Journal of virology, Vol. 63, Issue 11, pp. 4898-903, (1989) (PubMed).

Antigendetails

Target: Human Papilloma Virus 16 Capsid Protein (HPV-16 L1)

Andere Bezeichnung: HPV-16 L1

Substanzklasse: Viral Protein