FOXP1 Antikörper

Produktnummer ABIN2689508

Produktdetails anti-FOXP1 Antikörper

Target: FOXP1 (Forkhead Box P1 (FOXP1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FOXP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS)

Marke: BD Pharmingen™

Produktmerkmale: The JC12 monoclonal antibody specifically binds to Forkhead box protein P1 (FoxP1). FoxP1 belongs to subfamily P of the forkhead box (FOX) winged-helix transcription factor family. It plays a variety of roles such as regulating the formation of lymphoid, lung, brain and heart tissues. FoxP1 is critical in regulating early B cell development and is also required for maintenance of naive T cell quiescence, and monocyte differentiation and macrophage function. Aberrant expression of FOXP1 has been linked with mucosa-associated lymphoid tissue lymphoma and diffuse-large B cell lymphoma. Panel 1. Western blot analysis of FoxP1 expression in human Ramos cell lysate. Lysate prepared from cells of the human Ramos (Burkitt's lymphoma, ATCC CRL-1596) cell line was electrophoresed (SDS-PAGE), transferred to a polyvinylidene difluoride membrane, and Western blotted using Purified Anti-FoxP1 antibody (Cat. No. 564213, 2 μg/mL, overnight). Human Forkhead box protein P1 was identified as a band whose size ranged from 75-85 kDa. Panel 2. Immunohistochemical staining of acetone-fixed human tonsil for FoxP1. Acetone-fixed, frozen human tonsil sections were stained with either Purified Mouse IgG2a, κ Isotype Control (Cat. No. 554126) or Purified Mouse Anti-FoxP1 antibody. A three-step staining procedure using Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin-HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The FoxP1-specific antibody primarily stained some lymphocytes and epithelial cells with a nuclear pattern. Original magnification: 20X. Panel 3. Immunohistochemical staining of formalin-fixed human tonsil for FoxP1. Following antigen retrieval with BD Pharmingen™ Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG2a κ Isotype Control or Purified Mouse Anti-Human FoxP1 antibody. A three-step staining procedure was similarly used as before to develop the primary staining reagents. The FoxP1-specific antibody primarily stained some lymphocytes with a nuclear pattern. Original magnification: 40X.

BD Pharmingen™ Purified Mouse Anti-FoxP1 - Purified - Clone JC12 - Isotype Mouse IgG2a, κ - Reactivity Ms, Hu - 50 μg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Klon: JC12

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-FOXP1 Antikörper (ABIN2689508)

Sagardoy, Martinez-Ferrandis, Roa, Bunting, Aznar, Elemento, Shaknovich, Fontán, Fresquet, Perez-Roger, Robles, De Smedt, Sagaert, Melnick, Martinez-Climent: "Downregulation of FOXP1 is required during germinal center B-cell function." in: Blood, Vol. 121, Issue 21, pp. 4311-20, (2013) (PubMed).

Katoh, Igarashi, Fukuda, Nakagama, Katoh: "Cancer genetics and genomics of human FOX family genes." in: Cancer letters, Vol. 328, Issue 2, pp. 198-206, (2012) (PubMed).

Feng, Wang, Takata, Day, Willen, Hu: "Transcription factor Foxp1 exerts essential cell-intrinsic regulation of the quiescence of naive T cells." in: Nature immunology, Vol. 12, Issue 6, pp. 544-50, (2011) (PubMed).

Brown, Ashe, Leich, Burek, Barrans, Fenton, Jack, Pulford, Rosenwald, Banham: "Potentially oncogenic B-cell activation-induced smaller isoforms of FOXP1 are highly expressed in the activated B cell-like subtype of DLBCL." in: Blood, Vol. 111, Issue 5, pp. 2816-24, (2008) (PubMed).

Shi, Sakuma, Mooroka, Liscoe, Gao, Croce, Sharma, Kaplan, Greaves, Wang, Simon: "Down-regulation of the forkhead transcription factor Foxp1 is required for monocyte differentiation and macrophage function." in: Blood, Vol. 112, Issue 12, pp. 4699-711, (2008) (PubMed).

Hu, Wang, Borde, Nardone, Maika, Allred, Tucker, Rao: "Foxp1 is an essential transcriptional regulator of B cell development." in: Nature immunology, Vol. 7, Issue 8, pp. 819-26, (2006) (PubMed).

Streubel, Vinatzer, Lamprecht, Raderer, Chott: "T(3;14)(p14.1;q32) involving IGH and FOXP1 is a novel recurrent chromosomal aberration in MALT lymphoma." in: Leukemia, Vol. 19, Issue 4, pp. 652-8, (2005) (PubMed).

Wlodarska, Veyt, De Paepe, Vandenberghe, Nooijen, Theate, Michaux, Sagaert, Marynen, Hagemeijer, De Wolf-Peeters: "FOXP1, a gene highly expressed in a subset of diffuse large B-cell lymphoma, is recurrently targeted by genomic aberrations." in: Leukemia, Vol. 19, Issue 8, pp. 1299-305, (2005) (PubMed).

Wang, Lin, Li, Tucker: "Multiple domains define the expression and regulatory properties of Foxp1 forkhead transcriptional repressors." in: The Journal of biological chemistry, Vol. 278, Issue 27, pp. 24259-68, (2003) (PubMed).

Banham, Beasley, Campo, Fernandez, Fidler, Gatter, Jones, Mason, Prime, Trougouboff, Wood, Cordell: "The FOXP1 winged helix transcription factor is a novel candidate tumor suppressor gene on chromosome 3p." in: Cancer research, Vol. 61, Issue 24, pp. 8820-9, (2001) (PubMed).

Details zu FOXP1

Target: FOXP1 (Forkhead Box P1 (FOXP1))

Andere Bezeichnung: FoxP1 (FOXP1 Produkte)

Hintergrund: Synonyms: Forkhead box protein P1, hFKH1B, HSPC215, QRF1, 12CC4

Molekulargewicht: 75-85 kDa

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response