Fetal Hemoglobin Antikörper

Produktnummer ABIN2689504

Produktdetails anti-Fetal Hemoglobin Antikörper

Target: Fetal Hemoglobin (HbF)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fetal Hemoglobin Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: Reacts with fetal hemoglobin (HbF), a form of hemoglobin present during fetal development. HbF resembles adult hemoglobin (HbA) in possessing two alpha chains but differs in possessing two gamma instead of two beta chains. In normal adults, synthesis of HbF does persist at very low levels (<1 % of total Hb) and is restricted to a small population of erythrocytes called F cells. Hemoglobin F-expressing erythrocytes are normally seen during pregnancy. An increase in the expression of fetal hemoglobin in adult peripheral red blood cells is a common feature in the genetic disorders of hemoglobin, sickle-cell disease (SCD) and beta thalassemia. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of erythrocytes obtained from cord blood analyzed by flow cytometry. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-Human Fetal Hemoglobin - Purified - Clone 2D12 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Klon: 2D12

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-Fetal Hemoglobin Antikörper (ABIN2689504)

Campbell, Ware, Mason: "Detection of hemoglobin variants in erythrocytes by flow cytometry." in: Cytometry, Vol. 35, Issue 3, pp. 242-8, (1999) (PubMed).

Horiuchi, Osterhout, Kamma, Bekoe, Hirokawa: "Estimation of fetal hemoglobin levels in individual red cells via fluorescence image cytometry." in: Cytometry, Vol. 20, Issue 3, pp. 261-7, (1995) (PubMed).

Thorpe, Thein, Sampietro, Craig, Mahon, Huehns: "Immunochemical estimation of haemoglobin types in red blood cells by FACS analysis." in: British journal of haematology, Vol. 87, Issue 1, pp. 125-32, (1994) (PubMed).

Details zu Fetal Hemoglobin

Target: Fetal Hemoglobin (HbF)

Andere Bezeichnung: Fetal Hemoglobin (HbF Produkte)