CXCR5 Antikörper (Biotin)

Produktnummer ABIN2689484

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch CXCR5 in FACS. Er zeigt eine Reaktivität gegenüber Maus und wurde in 1 Publikation erwähnt.

Kurzübersicht für CXCR5 Antikörper (Biotin) (ABIN2689484)

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CXCR5 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: 2G8

Produktdetails anti-CXCR5 Antikörper (Biotin)

Marke: BD Pharmingen™

Produktmerkmale: The monoclonal antibody 2G8 reacts with the mouse CXC chemokine receptor, CXCR5. CXCR5 (a.k.a. BLR1, NLR and MDR15), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, CXCL13/BLC/BCA-1. The expression of CXCR5 has been detected in spleen, lymph nodes, tonsils, brain, bone marrow, T cells, B cells, cerebrum, cerebellum, hippcampus and pituitary. In mouse spleen, CXCR5 was strictly expressed by mature B cells and a small subset of T lymphocytes. The immunogen used to generate 2G8 hybridoma was a recombinant protein containing N-terminal amino acids of mouse CXCR5 (GST-NmBLR1). This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of CXCR5 expression on mouse splenocytes by biotinylated 2G8. Mouse splenocytes were stained with 1.0 μg/test of biotinylated 2G8, Streptavidin-PE (Cat. No. 554061) and anti-mouse CD45R/B220-FITC (Cat. No. 553088). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using biotinylated rat IgG2a (cat. No. 553928) as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control.

BD Pharmingen™ Biotin Rat Anti-Mouse CXCR5 - Biotin - Clone 2G8 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CXCR5

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-CXCR5 Antikörper (Biotin) (ABIN2689484)

Förster, Mattis, Kremmer, Wolf, Brem, Lipp: "A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen." in: Cell, Vol. 87, Issue 6, pp. 1037-47, (1997) (PubMed).

Details zu CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Andere Bezeichnung: CXCR5