CD63 Antikörper

Produktnummer ABIN2689369

Dieses Anti-CD63-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von CD63 in FACS, IF, BI und ICS. Geeignet für Maus. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für CD63 Antikörper (ABIN2689369)

Target: CD63

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD63 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)

Klon: NVG

Produktdetails anti-CD63 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The NVG-2 monoclonal antibody specifically binds to CD63 which is also known as Tspan30, Melanoma 1 antigen, ME491, and C75951. CD63 is a tetraspanin which belongs to the transmembrane-4 glycoprotein superfamily (TM4SF). It constitutes a major component of lysosomal membranes and translocates to the cell surface in response to various stimuli. CD63 is expressed by activated platelets, granulocytes, monocytes, macrophages, endothelium, fibroblasts, osteoblasts, and smooth muscle cells. CD63 can associate with other membrane molecules and functions in cellular signaling, adhesion and motility. Analyses of CD63 expression. Panel 1 and 2. Multicolor flow cytometric analysis of CD63 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927, Panel 1) or Purified Rat Anti-Mouse CD63 antibody (Cat. No. 564221, Panel 2). The cells were washed and counterstained with PE Goat Anti-Rat Ig (Cat. No. 550767). After washing, the cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 553129/561083). Two-color flow cytometric dot plots show the correlated expression patterns of Ly-6G and Ly-6C versus CD63 (or Ig Isotype control staining) for gated events with the forward and sid light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Panel 3. Immunofluorescent staining of CD63 in bEnd.3 cells. Adherent bEnd.3 cells (ATCC CRL-2299) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1 % Triton™ X-100 (Sigma, X100), and stained with either Purified Rat IgG2a, κ Isotype Control (Upper Panel 3) or Purified Rat Anti-Mouse CD63 antibody (Lower Panel 3). The cells were washed and then counterstained with Alexa Fluor® 488 Goat Anti-Rat IgG (H+L) Antibody (Life Technologies, Cat. No. A11006, pseudocolored green) and Hoechst 33342 Solution (Cat. No. 561908, pseudocolored blue). Images were analyzed using a BD Pathway™ 435 cell imager and merged using BD AttoVision™ Software.

BD Pharmingen™ Purified Rat Anti-Mouse CD63 - Purified - Clone NVG-2 - Isotype Rat IgG2a, κ - Reactivity Ms - 50 μg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Eosinophils

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD63 Antikörper (ABIN2689369)

Verjan Garcia, Umemoto, Saito, Yamasaki, Hata, Matozaki, Murakami, Jung, Woo, Seoh, Jang, Aozasa, Miyasaka: "SIRP?/CD172a regulates eosinophil homeostasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2268-77, (2011) (PubMed).

Miyamoto, Homma, Hotta: "Molecular cloning of the murine homologue of CD63/ME491 and detection of its strong expression in the kidney and activated macrophages." in: Biochimica et biophysica acta, Vol. 1217, Issue 3, pp. 312-6, (1994) (PubMed).

Details zu CD63

Target: CD63

Andere Bezeichnung: CD63

Hintergrund: Synonyms: Tspan30, Melanoma 1 antigen, ME491, C75951