NCR1 Antikörper

Produktnummer ABIN2689197

Der Ratte Monoklonal Anti-NCR1-Antikörper wurde für FACS validiert. Er ist geeignet, NCR1 in Proben von Maus zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für NCR1 Antikörper (ABIN2689197)

Target: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser NCR1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: 29A1-4

Produktdetails anti-NCR1 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The monoclonal antibody 29A1.4 specifically binds to mouse CD335, also known as NKp46. NKp46 is a 46 kDa type I transmembrane glycoprotein that is a member of the natural cytotoxicity receptor (NCR) family and immunoglobulin superfamily. NKp46 is encoded by the Ncr1 gene located on chromosome 7. NKp46 functions as a cytotoxicity triggering receptor and is selectively expressed by immature and mature NK cells in all mouse strains tested. NKp46 is detected on a minute fraction of NK-like T cells (less than 2 % of NKp46+ express CD3e) but not on CD1d-restricted NKT cells from C57BL/6 mice. When immobilized on tissue culture plates, the 29A1.4 antibody reportedly stimulates NK cells to produce interferon-gamma and to release their cytoplasmic granule contents. Although the ligands for the NKp46 receptor have not been fully characterized, recent evidence indicates that this receptor plays an important role in the NK cell-mediated recognition and killing of some virus-infected cells and tumor cells. The immunogen used to generate the 29A1.4 clone was mouse NKp46-Fc recombinant protein. Flow cytometric analysis of CD335 (NKp46) expression on mouse splenocytes. C57BL/6 and BALB/c mouse spleen cells were stained separately with Purified anti-mouse CD335 (NKp46) antibody followed by PE-conjugated Goat anti-rat Ig (Cat. No. 550767). After washing, C57BL/6 cells were stained with APC-conjugated anti-mouse NK-1.1 (NKR-P1B and NKR-P1C) antibody (Cat. No.550627, left panel) and BALB/c cells were stained with FITC-conjugated anti-mouse CD49b (DX5) antibody (Cat. No.553857, right panel). Two-color dot plots showing the correlated expression patterns of CD335/NKp46 and either NK-1.1/CD161 (C57BL/6 cells, left panel) or DX5/CD49b (BALB/c cells, right panel) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.

BD Pharmingen™ Purified Rat Anti-Mouse CD335 (NKp46) - Purified - Clone 29A1.4 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-NCR1 Antikörper (ABIN2689197)

Joncker, Fernandez, Treiner, Vivier, Raulet: "NK cell responsiveness is tuned commensurate with the number of inhibitory receptors for self-MHC class I: the rheostat model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 8, pp. 4572-80, (2009) (PubMed).

Walzer, Bléry, Chaix, Fuseri, Chasson, Robbins, Jaeger, André, Gauthier, Daniel, Chemin, Morel, Dalod, Imbert, Pierres, Moretta, Romagné, Vivier: "Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 9, pp. 3384-9, (2007) (PubMed).

Gazit, Gruda, Elboim, Arnon, Katz, Achdout, Hanna, Qimron, Landau, Greenbaum, Zakay-Rones, Porgador, Mandelboim: "Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1." in: Nature immunology, Vol. 7, Issue 5, pp. 517-23, (2006) (PubMed).

Biassoni, Pessino, Bottino, Pende, Moretta, Moretta: "The murine homologue of the human NKp46, a triggering receptor involved in the induction of natural cytotoxicity." in: European journal of immunology, Vol. 29, Issue 3, pp. 1014-20, (1999) (PubMed).

Details zu NCR1

Target: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Andere Bezeichnung: CD335 NKp46

Hintergrund: Synonyms: NKp46, Ar1, Ly94, Lymphocyte antigen 94, Mar1, MAR-1, Mouse activating rece

Pathways: Regulation of Leukocyte Mediated Immunity