CD28 Antikörper

Produktnummer ABIN2689147

Produktdetails anti-CD28 Antikörper

Target: CD28

Reaktivität: Pavian, Cynomolgus, Rhesusaffen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD28 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 Molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells. This clone also cross-reacts with a subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, rhesus and cynomolgus macaques. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes, however, as described in the literature, the frequency of CD28.2-positive lymphocytes is reduced in the non-human primate, with the majority of cells being CD28 negative. Profile of anti-CD28 reactivity on peripheral blood lymphocytes of Rhesus macaque (macaca mulatta) analyzed by flow cytometry.

BD Pharmingen™ Purified Mouse Anti-Human CD28 - Purified - Clone CD28.2 - Isotype Mouse IgG1, κ - Reactivity Bab, Cyno, Rhe - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CD28 Transfected Cell Line

Klon: CD28-2

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28 (CD28 Produkte)

Synonyme: Tp44 antikoerper, CD28RNA antikoerper, cd28 antikoerper, CD28 molecule antikoerper, CD28 antigen antikoerper, Cd28 molecule antikoerper, CD28 molecule L homeolog antikoerper, CD28 antikoerper, Cd28 antikoerper, cd28.L antikoerper

Hintergrund: Synonyms: CD28 antigen, T44, Tp44, TP44

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response