CD28 Antikörper

Produktnummer ABIN2689147

Der Maus Monoklonal anti-CD28 Antikörper (Klon CD28-2) (ABIN2689147) detektiert spezifisch CD28 in FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Pavian, Cynomolgus und Rhesusaffen.

Kurzübersicht für CD28 Antikörper (ABIN2689147)

Target: CD28

Reaktivität: Pavian, Cynomolgus, Rhesusaffen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD28 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: CD28-2

Produktdetails anti-CD28 Antikörper

Produktmerkmale: The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 Molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells. This clone also cross-reacts with a subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, rhesus and cynomolgus macaques. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes, however, as described in the literature, the frequency of CD28.2-positive lymphocytes is reduced in the non-human primate, with the majority of cells being CD28 negative. Profile of anti-CD28 reactivity on peripheral blood lymphocytes of Rhesus macaque (macaca mulatta) analyzed by flow cytometry.

BD Pharmingen™ Purified Mouse Anti-Human CD28 - Purified - Clone CD28.2 - Isotype Mouse IgG1, κ - Reactivity Bab, Cyno, Rhe - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CD28 Transfected Cell Line

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28

Hintergrund: Synonyms: CD28 antigen, T44, Tp44, TP44

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response