CD32/CD16 Antikörper

Produktnummer ABIN2689006

Produktdetails anti-CD32/CD16 Antikörper

Target: CD32/CD16

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD32/CD16 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: BD Pharmingen™

Produktmerkmale: The 2.4G2 antibody reacts specifically with a common nonpolymorphic epitope on the extracellular domains of the mouse FcγIII and FcγII receptors. It has also been reported to bind the FcγI receptor (CD64) via its Fc domain. 2.4G2 mAb blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII, and possibly FcγI, receptors in vitro and in vivo. CD16 and/or CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two color analysis of the expression of CD16/CD32 on mouse spleen cells and demonstration of FCγR-mediated non-specific staining. Left: BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. No. 553063/553064) and purified 2.4G2 mAb. The staining by 2.4G2 antibody was detected with FITC-conjugated mouse anti-rat lg, κ chain mAb MRK-1 (Cat. No. 553872). Right: BALB/c splenocytes were stained with FITC-conjugated rat anti-mouse CD90.2 (Thy-1.2) mAb 53-2.1 (Cat. No. 553003/553004) in the presence of purified 2.4G2 mAb (filled histogram) and without 2.4G2 mAb (open histogram). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) - Purified - Clone 2.4G2 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse BALB/c Macrophage J774 Cell Line

Klon: 2-4G2

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD32/CD16 Antikörper (ABIN2689006)

Kaji, Takeshita, Miyake, Takai, Kudo: "Functional association of CD9 with the Fc gamma receptors in macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3256-65, (2001) (PubMed).

Takezawa, Watanabe, Akaike: "Direct evidence of macrophage differentiation from bone marrow cells in the liver: a possible origin of Kupffer cells." in: Journal of biochemistry, Vol. 118, Issue 6, pp. 1175-83, (1996) (PubMed).

Araujo-Jorge, Rivera, el Bouhdidi, Daëron, Carlier: "An Fc gamma RII-, Fc gamma RIII-specific monoclonal antibody (2.4G2) decreases acute Trypanosoma cruzi infection in mice." in: Infection and immunity, Vol. 61, Issue 11, pp. 4925-8, (1993) (PubMed).

Rodewald, Awad, Moingeon, DAdamio, Rabinowitz, Shinkai, Alt, Reinherz: "Fc gamma RII/III and CD2 expression mark distinct subpopulations of immature CD4-CD8- murine thymocytes: in vivo developmental kinetics and T cell receptor beta chain rearrangement status." in: The Journal of experimental medicine, Vol. 177, Issue 4, pp. 1079-92, (1993) (PubMed).

Latour, Bonnerot, Fridman, Daëron: "Induction of tumor necrosis factor-alpha production by mast cells via Fc gamma R. Role of the Fc gamma RIII gamma subunit." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 6, pp. 2155-62, (1992) (PubMed).

Maeda, Burton, Padgett, Conrad, Huff, Masuda, Szakal, Tew: "Murine follicular dendritic cells and low affinity Fc receptors for IgE (Fc epsilon RII)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 8, pp. 2340-7, (1992) (PubMed).

Rodewald, Moingeon, Lucich, Dosiou, Lopez, Reinherz: "A population of early fetal thymocytes expressing Fc gamma RII/III contains precursors of T lymphocytes and natural killer cells." in: Cell, Vol. 69, Issue 1, pp. 139-50, (1992) (PubMed).

Vremec, Zorbas, Scollay, Saunders, Ardavin, Wu, Shortman: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, (1992) (PubMed).

Benhamou, Bonnerot, Fridman, Daëron: "Molecular heterogeneity of murine mast cell Fc gamma receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 8, pp. 3071-7, (1990) (PubMed).

Katz, Arm, Benson, Austen: "Maturation-related changes in the expression of Fc gamma RII and Fc gamma RIII on mouse mast cells derived in vitro and in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 10, pp. 3412-7, (1990) (PubMed).

Perussia, Tutt, Qiu, Kuziel, Tucker, Trinchieri, Bennett, Ravetch, Kumar: "Murine natural killer cells express functional Fc gamma receptor II encoded by the Fc gamma R alpha gene." in: The Journal of experimental medicine, Vol. 170, Issue 1, pp. 73-86, (1989) (PubMed).

Lewis, Koch, Plutner, Mellman: "A complementary DNA clone for a macrophage-lymphocyte Fc receptor." in: Nature, Vol. 324, Issue 6095, pp. 372-5, (1987) (PubMed).

Ravetch, Luster, Weinshank, Kochan, Pavlovec, Portnoy, Hulmes, Pan, Unkeless: "Structural heterogeneity and functional domains of murine immunoglobulin G Fc receptors." in: Science (New York, N.Y.), Vol. 234, Issue 4777, pp. 718-25, (1986) (PubMed).

Kurlander, Ellison, Hall: "The blockade of Fc receptor-mediated clearance of immune complexes in vivo by a monoclonal antibody (2.4G2) directed against Fc receptors on murine leukocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 2, pp. 855-62, (1984) (PubMed).

Witmer, Steinman: "The anatomy of peripheral lymphoid organs with emphasis on accessory cells: light-microscopic immunocytochemical studies of mouse spleen, lymph node, and Peyer's patch." in: The American journal of anatomy, Vol. 170, Issue 3, pp. 465-81, (1984) (PubMed).

Titus, Finkelman, Stephany, Jones, Segal: "Quantitative analysis of Fc gamma receptors on murine spleen cell populations by using dual parameter flow cytometry." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 2, pp. 556-61, (1984) (PubMed).

Mellman, Unkeless: "Purificaton of a functional mouse Fc receptor through the use of a monoclonal antibody." in: The Journal of experimental medicine, Vol. 152, Issue 4, pp. 1048-69, (1980) (PubMed).

Unkeless: "Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors." in: The Journal of experimental medicine, Vol. 150, Issue 3, pp. 580-96, (1979) (PubMed).

Details zu CD32/CD16

Target: CD32/CD16

Andere Bezeichnung: CD16,CD32 (CD32/CD16 Produkte)

Hintergrund: Synonyms: Fcγ III/II Receptor