CD16 Antikörper (Biotin)

Produktnummer ABIN2689005

Der Maus Monoklonal Anti-CD16-Antikörper wurde für FACS validiert. Er ist geeignet, CD16 in Proben von Schwein zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für CD16 Antikörper (Biotin) (ABIN2689005)

Target: CD16

Reaktivität: Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD16 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: FcG7

Produktdetails anti-CD16 Antikörper (Biotin)

Marke: BD Pharmingen™

Produktmerkmale: The FcG7 antibody reacts with CD16, the transmembrane α chain of Fcγ Receptor III (FcγRIIIAα). In the pig, FcγRIIIAα is expressed on NK cells, monocytes, granulocytes, and pulmonary alveolar macrophages. The FcG7 antibody induces redirected cytotoxic activity of NK cells and granulocytes against tumor cells. Two-color analysis of CD16 expression on pig peripheral blood lymphocytes. Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 (Cat. no. 559586, both panels) and biotinylated mAb FcG7 (Right panel), followed by Streptavidin- FITC (Cat. No. 554060, both panels). Erythrocytes were lysed (PharmLyse™ Lysing Buffer, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via- Probe™, Cat. No. 555816/555815), and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCalibura .

BD Pharmingen™ Biotin Mouse Anti-Pig CD16 - Biotin - Clone FcG7 - Isotype Mouse IgG1, κ - Reactivity Pig - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Pig peripheral blood leukocytes

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD16 Antikörper (Biotin) (ABIN2689005)

Aller, Cho, Kim: "Characterization of the cytolytic trigger molecules G7/PNK-E as a molecular complex on the surface of porcine phagocytes." in: Cellular immunology, Vol. 161, Issue 2, pp. 270-8, (1995) (PubMed).

Halloran, Sweeney, Strohmeier, Kim: "Molecular cloning and identification of the porcine cytolytic trigger molecule G7 as a Fc gamma RIII alpha (CD16) homologue." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 6, pp. 2631-41, (1994) (PubMed).

Halloran, Sweeney, Kim: "Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7." in: Cellular immunology, Vol. 158, Issue 2, pp. 400-13, (1994) (PubMed).

Dato, Wierda, Kim: "A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes." in: Cellular immunology, Vol. 140, Issue 2, pp. 468-77, (1992) (PubMed).

Details zu CD16

Target: CD16

Andere Bezeichnung: CD16

Hintergrund: Synonyms: Fcγ Receptor IIIAα

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process