Asialoglycoprotein Receptor 1 Antikörper

Produktnummer ABIN2688844

Dieses Anti-Asialoglycoprotein Receptor 1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Asialoglycoprotein Receptor 1 in WB, IF, FACS und BI. Geeignet für Human und Ratte. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für Asialoglycoprotein Receptor 1 Antikörper (ABIN2688844)

Target: Asialoglycoprotein Receptor 1 (ASGR1)

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Asialoglycoprotein Receptor 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), BioImaging (BI)

Klon: 8D7

Produktdetails anti-Asialoglycoprotein Receptor 1 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: The 8D7 monoclonal antibody recognizes Asialoglycoprotein receptor 1 (ASGPR 1), also known as Hepatic lectin H1 (HL-1). ASGPR 1 is an approximately 42 kDA type II integral membrane protein that is expressed on the surface of hepatic cells. It is expressed by hepatocytes on the sinusoidal-lateral plasma membrane but not on the bile canalicular membrane. ASGPR 1 plays a role in serum glycoprotein homeostasis. It functions as a subunit of the Asialoglycoprotein receptor (ASGPR) complex that binds, internalizes, and transports various glycoproteins for lysosomal degradation. The receptor may also promote hepatic infection by the binding and uptake of various viruses. The immunogen used to generate the 8D7 hybridoma was rat liver membrane extracts. Rat ASGPR consists of three polypeptide subunits (Rat hepatic lectin 1-3 (RHL1-3). The 8D7 antibody has been shown to react with a subunit-specific epitope on RHL-1.

BD Pharmingen™ Purified Mouse Anti-ASGPR 1 - Purified - Clone 8D7 - Isotype Mouse IgG1, κ - Reactivity Hu, Rat - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Liver membrane constituents

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry System. Immunofluorescent Staining of ASGPR 1 in Human Hepatocellular Carcinoma (Hep G2) cells. Hep G2 cells (ATCC, HB-8065) were fixed with BD Cytofix™ Fixation Buffer and stained with Purified Mouse Anti-ASGPR 1 (pseudo-colored green) at 5 μg/mL. The second-step reagent was Alexa Fluor® 488 Goat Anti-Mouse IgG (Life Technologies), and counter-staining was with DAPI (4',6-Diamidino-2-phenylindole dihydrochloride, pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-Asialoglycoprotein Receptor 1 Antikörper (ABIN2688844)

Touboul, Hannan, Corbineau, Martinez, Martinet, Branchereau, Mainot, Strick-Marchand, Pedersen, Di Santo, Weber, Vallier: "Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development." in: Hepatology (Baltimore, Md.), Vol. 51, Issue 5, pp. 1754-65, (2010) (PubMed).

Shimada, Mizuno, Uesu, Nasu, Okada, Shimomura, Yamamoto, Tsuji, Shiratori: "A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 26, Issue 1, pp. 55-60, (2003) (PubMed).

Mizuno, Yamada, Nagashima: "Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes." in: Gastroenterologia Japonica, Vol. 21, Issue 3, pp. 238-44, (1986) (PubMed).

Details zu Asialoglycoprotein Receptor 1

Target: Asialoglycoprotein Receptor 1 (ASGR1)

Andere Bezeichnung: ASGPR 1

Hintergrund: Synonyms: Asialoglycoprotein receptor 1, ASGR1, ASGP-R1, ASGPR 1, HL-1, CLEC4H1

Pathways: Thyroid Hormone Synthesis