EZH2 Antikörper (AA 353-451)

Produktnummer ABIN7938124

Zitiert in 8+ Publikationen. Der Maus Monoklonal anti-EZH2 Antikörper (Klon AC22) (ABIN7938124) detektiert spezifisch EZH2 in ChIP. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für EZH2 Antikörper (AA 353-451) (ABIN7938124)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EZH2 Antikörper ist unkonjugiert

Applikation: Chromatin Immunoprecipitation (ChIP)

Klon: AC22

Produktdetails anti-EZH2 Antikörper

Bindungsspezifität: AA 353-451

Verwendungszweck: EZH2 antibody (mAb) (Clone AC22)

Aufreinigung: Protein A Chromatography

Immunogen: This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ChIP: 1 - 3 μg per ChIP CUT&RUN: 1 µL per 50 µL reaction This antibody has been published, but not validated by Active Motif, for Western blot. For an antibody we have tested in use in Western blotting, see Catalog No. 39933. For EZH2, we also offer an AbFlex EZH2 Recombinant Antibody (rAb). For details, see Catalog No. 91159.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-EZH2 Antikörper (ABIN7938124)

Toth, Papp, Brulois, Choi, Gao, Jung: "LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection." in: PLoS pathogens, Vol. 12, Issue 9, pp. e1005878, (2016) (PubMed).

Ranzani, Rossetti, Panzeri, Arrigoni, Bonnal, Curti, Gruarin, Provasi, Sugliano, Marconi, De Francesco, Geginat, Bodega, Abrignani, Pagani: "The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4." in: Nature immunology, Vol. 16, Issue 3, pp. 318-325, (2015) (PubMed).

Bengani, Mendiratta, Maini, Vasanthi, Sultana, Ghasemi, Ahluwalia, Ramachandran, Mishra, Brahmachari: "Identification and Validation of a Putative Polycomb Responsive Element in the Human Genome." in: PLoS ONE, Vol. 8, Issue 6, pp. e67217, (2013) (PubMed).

Wang, Pan, Lee, Martinez, Feng, Dong: "Cutting edge: Smad2 and Smad4 regulate TGF-β-mediated Il9 gene expression via EZH2 displacement." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 10, pp. 4908-12, (2013) (PubMed).

Rossetto, Tarrant-Elorza, Pari: "Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells." in: PLoS pathogens, Vol. 9, Issue 5, pp. e1003366, (2013) (PubMed).

Lin, Shen, Li, Tang, Gu, Chen, Hu, Rice, Lu, Wu: "Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program." in: The Journal of biological chemistry, Vol. 288, Issue 9, pp. 6238-47, (2013) (PubMed).

Woellmer, Arteaga-Salas, Hammerschmidt: "BZLF1 governs CpG-methylated chromatin of Epstein-Barr Virus reversing epigenetic repression." in: PLoS pathogens, Vol. 8, Issue 9, pp. e1002902, (2012) (PubMed).

Murata, Kondo, Sugimoto, Kawashima, Saito, Isomura, Kanda, Tsurumi: "Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells." in: Journal of virology, Vol. 86, Issue 9, pp. 4752-61, (2012) (PubMed).

Details zu EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Andere Bezeichnung: EZH2

Hintergrund: EZH2 - (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

Molekulargewicht: 96 kDa

Gen-ID: 2146

NCBI Accession: NP_004447

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation