SUZ12 Antikörper

Produktnummer ABIN2668948

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-SUZ12 Antikörper wird verwendet zum Nachweis von SUZ12 in Proben von Human. Er wurde validiert für IHC, IP und ChIP.

Kurzübersicht für SUZ12 Antikörper (ABIN2668948)

Target: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SUZ12 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Klon: 2AO9

Produktdetails anti-SUZ12 Antikörper

Verwendungszweck: Suz12 antibody (mAb) (Clone 2AO9)

Aufreinigung: Protein G Chromatography

Immunogen: This Suz12 antibody was raised against a recombinant protein corresponding to full-length human Suz12.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ChIP: 2.5 - 10 μg per ChIP This antibody has been published, but has not been validated by Active Motif, for Western blot. For an antibody validated for use in Western blotting, see Catalog No. 39357. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91163.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-SUZ12 Antikörper (ABIN2668948)

Pasini, Hansen, Christensen, Agger, Cloos, Helin: "Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2." in: Genes & development, Vol. 22, Issue 10, pp. 1345-55, (2008) (PubMed).

Herranz, Pasini, Díaz, Francí, Gutierrez, Dave, Escrivà, Hernandez-Muñoz, Di Croce, Helin, García de Herreros, Peiró: "Polycomb complex 2 is required for E-cadherin repression by the Snail1 transcription factor." in: Molecular and cellular biology, Vol. 28, Issue 15, pp. 4772-81, (2008) (PubMed).

Details zu SUZ12

Target: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Andere Bezeichnung: Suz12

Hintergrund: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.

Molekulargewicht: 95 kDa

Gen-ID: 23512

NCBI Accession: NP_056170