Histone H4 Antikörper (H4K16ac)

Produktnummer ABIN2668860

Dieser Maus Monoklonal Antikörper detektiert spezifisch Histone H4 in WB, IF, ChIP und DB. Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für Histone H4 Antikörper (H4K16ac) (ABIN2668860)

Target: Histone H4 (HISTH4)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Klon: MABI 0416

Produktdetails anti-Histone H4 Antikörper

Bindungsspezifität: H4K16ac

Verwendungszweck: Histone H4K16ac antibody (mAb) (Clone MABI 0416)

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide containing acetyl-lysine 16 of human Histone H4.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ChIP: 2 - 5 μg per ChIP WB*: 0.5 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.7 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol, 0.3 M NaCl, and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone H4 Antikörper (ABIN2668860)

Meisenberg, Ashour, El-Shafie, Liao, Hodgson, Pilborough, Khurram, Downs, Ward, El-Khamisy: "Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan." in: Nucleic acids research, Vol. 45, Issue 3, pp. 1159-1176, (2017) (PubMed).

Details zu Histone H4

Target: Histone H4 (HISTH4)

Andere Bezeichnung: Histone H4

Hintergrund: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. NoRC is a SMARCA5 (SNF2h)-containing chromatin remodeling complex. The bromodomain of TIP5, the large subunit of NoRC, interacts with acetylated Histone H4 Lys16, (H4K16ac) and cooperates with an adjacent PHD finger to recruit histone deacetylases and DNA methyltransferases to rDNA, leading to the silencing of rDNA.

Molekulargewicht: 8 kDa

Gen-ID: 121504

NCBI Accession: NP_778224