Histone H4 Antikörper

Produktnummer ABIN2668856

Zitiert in 2+ Publikationen. Der Maus Monoklonal anti-Histone H4 Antikörper (Klon MABI 0400) (ABIN2668856) detektiert spezifisch Histone H4 in WB und ChIP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Histone H4 Antikörper (ABIN2668856)

Target: Histone H4 (HISTH4)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Klon: MABI 0400

Produktdetails anti-Histone H4 Antikörper

Verwendungszweck: Histone H4 antibody (mAb) (Clone MABI 0400)

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide containing human Histone H4.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ChIP: 2 - 5 μg per ChIP WB*: 0.5 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For Histone H4, we also offer AbFlex Histone H4 Recombinant Antibody (rAb). For details, see Catalog No. 91295.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.6 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol. 0.21M NaCl, and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone H4 Antikörper (ABIN2668856)

Bulusu, Tumanov, Michalopoulou, van den Broek, MacKay, Nixon, Dhayade, Schug, Vande Voorde, Blyth, Gottlieb, Vazquez, Kamphorst: "Acetate Recapturing by Nuclear Acetyl-CoA Synthetase 2 Prevents Loss of Histone Acetylation during Oxygen and Serum Limitation." in: Cell reports, Vol. 18, Issue 3, pp. 647-658, (2017) (PubMed).

Powers, Parvanov, Baker, Walker, Petkov, Paigen: "The Meiotic Recombination Activator PRDM9 Trimethylates Both H3K36 and H3K4 at Recombination Hotspots In Vivo." in: PLoS genetics, Vol. 12, Issue 6, pp. e1006146, (2016) (PubMed).

Details zu Histone H4

Target: Histone H4 (HISTH4)

Andere Bezeichnung: Histone H4

Hintergrund: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.

Molekulargewicht: 8 kDa

Gen-ID: 121504

NCBI Accession: NP_778224