Histone H4 Antikörper (H4K20me3)

Produktnummer ABIN2668855

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-Histone H4 Antikörper wird verwendet zum Nachweis von Histone H4 in Proben von Human und Maus. Er wurde validiert für WB, ICC, ChIP und DB.

Kurzübersicht für Histone H4 Antikörper (H4K20me3) (ABIN2668855)

Target: Histone H4 (HISTH4)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Klon: 6F8-D9

Produktdetails anti-Histone H4 Antikörper

Bindungsspezifität: H4K20me3

Verwendungszweck: Histone H4K20me3 antibody (mAb) (Clone 6F8-D9)

Aufreinigung: Protein G Chromatography

Immunogen: This Histone H4 trimethyl Lys20 antibody (mAb) was raised against a peptide containing trimethyl Lys20 of Histone H4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ChIP: 5 - 10 μg per ChIP WB*: 1 - 2 μg/mL dilution DB: 0.2 μg/mL dilution Slight reactivity towards histone H4 dimethyl Lys20 might be observed under certain conditions. Individual optimization may be required. *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer can be used to aid in detection by Western blot. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS with 0.035 % sodium azide and 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone H4 Antikörper (ABIN2668855)

Gonzalez-Vasconcellos, Schneider, Anastasov, Alonso-Rodriguez, Sanli-Bonazzi, Fernández, Atkinson: "The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression." in: Scientific reports, Vol. 7, pp. 42056, (2017) (PubMed).

Dal-Pra, Hodgkinson, Mirotsou, Kirste, Dzau: "Demethylation of H3K27 Is Essential for the Induction of Direct Cardiac Reprogramming by miR Combo." in: Circulation research, Vol. 120, Issue 9, pp. 1403-1413, (2017) (PubMed).

Nelson, Jaber-Hijazi, Cole, Robertson, Pawlikowski, Norris, Criscione, Pchelintsev, Piscitello, Stong, Rai, McBryan, Otte, Nixon, Clark, Riethman, Wu, Schotta, Garcia, Neretti, Baird, Berger, Adams: "Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability." in: Genome biology, Vol. 17, Issue 1, pp. 158, (2016) (PubMed).

Details zu Histone H4

Target: Histone H4 (HISTH4)

Andere Bezeichnung: Histone H4

Hintergrund: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.

Molekulargewicht: 8 kDa

Gen-ID: 121504

NCBI Accession: NP_778224