Histone H4 Antikörper (meLys20)

Produktnummer ABIN2668854

Dieser Maus Monoklonal Antikörper detektiert spezifisch Histone H4 in WB, IF, ICC, ChIP, DB und ChIP-seq. Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für Histone H4 Antikörper (meLys20) (ABIN2668854)

Target: Histone H4 (HISTH4)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Klon: 5E10-D8

Produktdetails anti-Histone H4 Antikörper

Bindungsspezifität: meLys20

Verwendungszweck: Histone H4K20me1 antibody (mAb) (Clone 5E10-D8)

Aufreinigung: Protein A Chromatography

Immunogen: This Histone H4 monomethyl Lys20 was raised against a synthetic peptide containing monomethyl Lys20 of Histone H4.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each ICC/IF: 1 μg/mL dilution WB: 0.5 - 2 μg/mL dilution DB: 1 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. For Histone H4K20me1, we also offer AbFlex Histone H4K20me1 Recombinant Antibody (rAb). For details, see Catalog No. 91291.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: PBS containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone H4 Antikörper (ABIN2668854)

Brasa, Mueller, Jacquemont, Hahne, Rozenberg, Peters, He, McCormack, Gasparini, Chibout, Grenet, Moggs, Gomez-Mancilla, Terranova: "Reciprocal changes in DNA methylation and hydroxymethylation and a broad repressive epigenetic switch characterize FMR1 transcriptional silencing in fragile X syndrome." in: Clinical epigenetics, Vol. 8, pp. 15, (2016) (PubMed).

Details zu Histone H4

Target: Histone H4 (HISTH4)

Andere Bezeichnung: Histone H4

Hintergrund: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression. Monomethylation of histone H4 at lysine 20 is detected on the inactive X chromosome in female mammals and implicated in chromosome condensation.

Molekulargewicht: 8 kDa

Gen-ID: 121504

NCBI Accession: NP_778224