SRF Antikörper

Produktnummer ABIN2668813

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Ratte Monoklonal anti-SRF Antikörper wird verwendet zum Nachweis von SRF in Proben von Human und Maus. Er wurde validiert für WB, IHC, IF, ChIP und ChIP-seq.

Kurzübersicht für SRF Antikörper (ABIN2668813)

Target: SRF (Serum Response Factor (SRF))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser SRF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Klon: 2C5

Produktdetails anti-SRF Antikörper

Verwendungszweck: SRF antibody (mAb) (Clone 2C5)

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a full-length protein corresponding to mouse SRF.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ChIP: 5 - 10 µL per ChIP ChIP-Seq: 5 - 10 µL each ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-SRF Antikörper (ABIN2668813)

Werfel, Jungmann, Lehmann, Ksienzyk, Bekeredjian, Kaya, Leuchs, Nordheim, Backs, Engelhardt, Katus, Müller: "Rapid and highly efficient inducible cardiac gene knockout in adult mice using AAV-mediated expression of Cre recombinase." in: Cardiovascular research, Vol. 104, Issue 1, pp. 15-23, (2014) (PubMed).

Koegel, von Tobel, Schäfer, Alberti, Kremmer, Mauch, Hohl, Wang, Beer, Bloch, Nordheim, Werner: "Loss of serum response factor in keratinocytes results in hyperproliferative skin disease in mice." in: The Journal of clinical investigation, Vol. 119, Issue 4, pp. 899-910, (2009) (PubMed).

Details zu SRF

Target: SRF (Serum Response Factor (SRF))

Andere Bezeichnung: SRF

Hintergrund: SRF (serum response factor) is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Molekulargewicht: 67 kDa

Gen-ID: 20807

NCBI Accession: NP_065239