HDAC1 Antikörper (AA 1-5, AA 433-448, AA 467-482)

Produktnummer ABIN2668768

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-HDAC1 Antikörper wird verwendet zum Nachweis von HDAC1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ChIP und ChIP-seq.

Kurzübersicht für HDAC1 Antikörper (AA 1-5, AA 433-448, AA 467-482) (ABIN2668768)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HDAC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-HDAC1 Antikörper

Bindungsspezifität: AA 1-5, AA 433-448, AA 467-482

Verwendungszweck: HDAC1 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This HDAC1 antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 1-5, 433-448 and 467-482 of human HDAC1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Validated Applications: ChIP: 4 μg per ChIP ChIP-Seq: 4 μg each WB: 1 - 2 μg/mL dilution For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-HDAC1 Antikörper (ABIN2668768)

Xiong, Svingen, Sarmento, Smyrk, Dave, Khanna, Lomberk, Urrutia, Faubion: "Differential coupling of KLF10 to Sin3-HDAC and PCAF regulates the inducibility of the FOXP3 gene." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 307, Issue 6, pp. R608-20, (2014) (PubMed).

Details zu HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Andere Bezeichnung: HDAC1

Hintergrund: HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

Molekulargewicht: 60 kDa

Gen-ID: 3065

NCBI Accession: NP_004955

Pathways: Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis