CHD5 Antikörper (C-Term)

Produktnummer ABIN2668553

Der Ratte Monoklonal anti-CHD5 Antikörper (Klon 5A10) (ABIN2668553) detektiert spezifisch CHD5 in WB, ICC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für CHD5 Antikörper (C-Term) (ABIN2668553)

Target: CHD5 (Chromodomain Helicase DNA Binding Protein 5 (CHD5))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CHD5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: 5A10

Produktdetails anti-CHD5 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Chd5 antibody (mAb) (Clone 5A10)

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a peptide within the C-terminal region of mouse Chd5.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ICC/IF: 2 - 5 μg/mL dilution WB*: 1 - 2 μg/mL dilution The addition of 0.1 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu CHD5

Target: CHD5 (Chromodomain Helicase DNA Binding Protein 5 (CHD5))

Andere Bezeichnung: Chd5

Hintergrund: Chd5 (Chromodomain Helicase DNA Binding Protein 5) is member of the CHD subfamily of chromatin remodeling SWI/SNF proteins, containing chromodomains and a SNF2-like ATPase/helicase domain, that play a role in chromatin remodeling and gene transcription. Chd5 is also a potential tumor suppressor that may play a role in neuronal development.

Molekulargewicht: 260 kDa

Gen-ID: 269610

NCBI Accession: NP_001074845